Latex allergy studies: extraction of natural rubber latex proteins with reference to film thickness latex d.r.c. and protein migration behaviour

Yeang H. Y., . and Sunderasan E., . and Hafsah Mohd Ghazaly, . Latex allergy studies: extraction of natural rubber latex proteins with reference to film thickness latex d.r.c. and protein migration behaviour. pp. 46-62. ISSN 0127-7065

Water-soluble proteins in wet natural rubber latex films co-migrated with the evaporation stream to the surface when the film was dried at 100C and moisture allowed to evaporate from one surface. When evaporation occurred from both surfaces of the latex film simultaneously the evaporative pull from both directions appeared to annul each other resulting in little protein migration to either surface. Since unmigrated proteins are not readily extracted the problem of allergenic proteins in latex films relates essentially to the proteins that migrate to the surface. Wet-gel leaching of thin latex films (0.15 mm thick when dry) and thicker films (0.25 -0.35 mm) to remove soluble proteins was investigated by gel-leaching the films for 1-3 min with distilled water at room temperature after which they were completely dried at 100C. Soluble proteins were reduced in thin films that were gel-leached. However gel-leaching was counter-productive for the thicker films where extractable proteins increased. The amount of proteins extractable from latex films was also influenced by the d. r. c. of the latex used to prepare the films. Films prepared from 40 d.r.c. latex had significantly higher extractable proteins than films of similar thickness that were prepared from 60 d.r.c. latex. Protein removal by wet-gel leaching was found not to be very effective because much of these proteins had not yet migrated to the surface when the film was heated briefly to attain the wet-gel state. When the wet-gel film was completely dried by prolonged heating after the leaching step more proteins migrated to the surface. In the process of even brief (3 min or less) dry-film leaching on the other hand most of the soluble proteins had evidently migrated to the film surface at the time of leaching and their removal was hence much more effective.

Full text available from: Official URL: http://vitaldoc.lgm.gov.my:8060/vital/access/servi...

Abstract

Water-soluble proteins in wet natural rubber latex films co-migrated with the evaporation stream to the surface when the film was dried at 100C and moisture allowed to evaporate from one surface. When evaporation occurred from both surfaces of the latex film simultaneously the evaporative pull from both directions appeared to annul each other resulting in little protein migration to either surface. Since unmigrated proteins are not readily extracted the problem of allergenic proteins in latex films relates essentially to the proteins that migrate to the surface. Wet-gel leaching of thin latex films (0.15 mm thick when dry) and thicker films (0.25 -0.35 mm) to remove soluble proteins was investigated by gel-leaching the films for 1-3 min with distilled water at room temperature after which they were completely dried at 100C. Soluble proteins were reduced in thin films that were gel-leached. However gel-leaching was counter-productive for the thicker films where extractable proteins increased. The amount of proteins extractable from latex films was also influenced by the d. r. c. of the latex used to prepare the films. Films prepared from 40 d.r.c. latex had significantly higher extractable proteins than films of similar thickness that were prepared from 60 d.r.c. latex. Protein removal by wet-gel leaching was found not to be very effective because much of these proteins had not yet migrated to the surface when the film was heated briefly to attain the wet-gel state. When the wet-gel film was completely dried by prolonged heating after the leaching step more proteins migrated to the surface. In the process of even brief (3 min or less) dry-film leaching on the other hand most of the soluble proteins had evidently migrated to the film surface at the time of leaching and their removal was hence much more effective.

[error in script]

Item Type:	Article
AGROVOC Term:	Natural rubber
AGROVOC Term:	Latex
AGROVOC Term:	Allergies
AGROVOC Term:	Thickness
AGROVOC Term:	Protein content
AGROVOC Term:	Evaporation
AGROVOC Term:	Allergens
AGROVOC Term:	Leaching
AGROVOC Term:	Distilled water
AGROVOC Term:	Drying
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 03:57
URI:	http://webagris.upm.edu.my/id/eprint/23381

Actions (login required)

View Item