Citation
Cardosa M. J., . and Sharifah Hamid, . and Samuel-Verghese S., . and Yeang Andhy, . Monoclonal antibodies identify different antigens in proteins eluted from natural rubber latex gloves obtained from different sources. pp. 270-277. ISSN 0127-7065
Abstract
An indirect enzyme linked immunosorbent assay (ELISA) was used to show that proteins eluted from different samples of natural rubber latex gloves contained different relative proportions of various antigens as defined by monoclonal antibodies directed against B-serum and C-serum proteins. Thus some gloves with low total protein content were shown to have a high proportion of one of these antigens but negligible amounts of other antigens while some other gloves had high levels of more than one antigen tested. These differences become important if some of these antigens are more allergenic than others and this report highlights the inadequacy of total protein quantitation in the absence of specific immunoassays as a measure of the safety of a product.
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Abstract
An indirect enzyme linked immunosorbent assay (ELISA) was used to show that proteins eluted from different samples of natural rubber latex gloves contained different relative proportions of various antigens as defined by monoclonal antibodies directed against B-serum and C-serum proteins. Thus some gloves with low total protein content were shown to have a high proportion of one of these antigens but negligible amounts of other antigens while some other gloves had high levels of more than one antigen tested. These differences become important if some of these antigens are more allergenic than others and this report highlights the inadequacy of total protein quantitation in the absence of specific immunoassays as a measure of the safety of a product.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Monoclonal antibodies |
AGROVOC Term: | Antigens |
AGROVOC Term: | Natural rubber |
AGROVOC Term: | Latex |
AGROVOC Term: | ELISA |
AGROVOC Term: | Food allergies |
AGROVOC Term: | Centrifugation |
AGROVOC Term: | Antiserum |
AGROVOC Term: | Hevea brasiliensis |
AGROVOC Term: | Hydrogen peroxide |
Geographical Term: | Malaysia |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 28 Apr 2025 04:31 |
URI: | http://webagris.upm.edu.my/id/eprint/23424 |
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