Citation
Shanmugam B., . and Muniandy S., . Effect of reduced glutathione and oxidised glutathione on the appearance of multiple forms of glutathione S-transferases in Hevea brasiliensis latex. pp. 136-141. ISSN 0127-7065
Abstract
Glutathione S-transferase in latex of Hevea brasiliensis appears to be a single protein containing presumably several labile disulphide bonds. Partial cleavage of these bonds occurs when the enzyme is eluted through a DEAE-Sephadex column in the presence of a sodium chloride gradient thus resulting in multiple forms. Glutathione but not oxidised glutathione (GSSG) in the eluting medium enhances the reduction process during ion-exchange chromatography. Reduced forms of the enzyme when dialysed free of sodium or sodium chloride plus the reducing agent after ion-exchange chromatography result in the formation of a major oxidised form with an isoelectric point similar to that of the crude enzyme.
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Abstract
Glutathione S-transferase in latex of Hevea brasiliensis appears to be a single protein containing presumably several labile disulphide bonds. Partial cleavage of these bonds occurs when the enzyme is eluted through a DEAE-Sephadex column in the presence of a sodium chloride gradient thus resulting in multiple forms. Glutathione but not oxidised glutathione (GSSG) in the eluting medium enhances the reduction process during ion-exchange chromatography. Reduced forms of the enzyme when dialysed free of sodium or sodium chloride plus the reducing agent after ion-exchange chromatography result in the formation of a major oxidised form with an isoelectric point similar to that of the crude enzyme.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Hevea brasiliensis |
| AGROVOC Term: | Hevea rubber |
| AGROVOC Term: | Latex |
| AGROVOC Term: | Glutathione |
| AGROVOC Term: | Oxidation |
| AGROVOC Term: | Plant protein |
| AGROVOC Term: | Enzyme activity |
| AGROVOC Term: | Ion exchange chromatography |
| AGROVOC Term: | Isoelectric focusing |
| AGROVOC Term: | Gsh |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 05:14 |
| URI: | http://webagris.upm.edu.my/id/eprint/23553 |
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