Citation
Samsidar Hamzah, . and Gomez J. B., . and Ho L. H., . A refinement of the staining techniques for Hevea latex vessels. pp. 163-166. ISSN 0127-7065
Abstract
A refined method to enhance the staining of Hevea latex vessels is described. Tissues were prepared following procedures for electron microscopy. Semi-thin sections (1-2 pm thick) were double-stained with aqueous safranin and 1 malachite green. The contents of latex vessels were stained cyclamen (purple) and their cell walls capri blue. The contents of tannin cells stained dark blue. These temporary preparations were good for colour photography for a few days. Comparison is made with the conventional staining affixed or resin-embedded material.
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Abstract
A refined method to enhance the staining of Hevea latex vessels is described. Tissues were prepared following procedures for electron microscopy. Semi-thin sections (1-2 pm thick) were double-stained with aqueous safranin and 1 malachite green. The contents of latex vessels were stained cyclamen (purple) and their cell walls capri blue. The contents of tannin cells stained dark blue. These temporary preparations were good for colour photography for a few days. Comparison is made with the conventional staining affixed or resin-embedded material.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Hevea |
| AGROVOC Term: | Hevea rubber |
| AGROVOC Term: | Latex |
| AGROVOC Term: | Plant tissues |
| AGROVOC Term: | Bark extracts |
| AGROVOC Term: | Electron microscopy |
| AGROVOC Term: | Formalin |
| AGROVOC Term: | Alcohols |
| AGROVOC Term: | Colour |
| AGROVOC Term: | Glycerin |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 05:14 |
| URI: | http://webagris.upm.edu.my/id/eprint/23556 |
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