Citation
Raman R., . and Muniandy S., . and Nembau H., . and Shanmugam B., . and Narasiman M., . The use of bromosulphonphthalein glutathione as an affinity ligand for the partial purification of GSH S-transferase from the latex of Hevea brasiliensis. pp. 183-190. ISSN 0127-7065
Abstract
Glutathione S-transferase from the latex of Hevea brasiliensis can be purified in large quantities using bromosulphonphthalein-glutathione as an affinity ligand. The fold of purification is affected by pH flow rate and the volume of enzyme eluted through the column. High folds of purification can be obtained-at a flow rate of approximately 14 ml per hour at pH 9.0 using large volumes of crude enzyme. The maximum specific activity so far obtained is in the region of 214 umoles per minute per milligramme protein resulting in a 832-fold purification. This method can now be adapted commercially for the purification of latex glutathione S-transferase in large quantities.
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Abstract
Glutathione S-transferase from the latex of Hevea brasiliensis can be purified in large quantities using bromosulphonphthalein-glutathione as an affinity ligand. The fold of purification is affected by pH flow rate and the volume of enzyme eluted through the column. High folds of purification can be obtained-at a flow rate of approximately 14 ml per hour at pH 9.0 using large volumes of crude enzyme. The maximum specific activity so far obtained is in the region of 214 umoles per minute per milligramme protein resulting in a 832-fold purification. This method can now be adapted commercially for the purification of latex glutathione S-transferase in large quantities.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Hevea brasiliensis |
| AGROVOC Term: | Latex |
| AGROVOC Term: | Glutathione |
| AGROVOC Term: | Gsh |
| AGROVOC Term: | Transferases |
| AGROVOC Term: | affinity groups |
| AGROVOC Term: | Ligands |
| AGROVOC Term: | Protein content |
| AGROVOC Term: | Enzyme activity |
| AGROVOC Term: | Purification |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 05:15 |
| URI: | http://webagris.upm.edu.my/id/eprint/23584 |
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