Assessment of antioxidant potential of phenolic compounds fractions of Algerian Ceratonia siliqua L. pods during ripening stages

Richard G., . and Chaalal M., . and López-Nicolás R., . and Fauconnier M. L., . and Ydjedd S., . and Louaileche H., . and Kati D. E., . Assessment of antioxidant potential of phenolic compounds fractions of Algerian Ceratonia siliqua L. pods during ripening stages. pp. 2041-2049. ISSN 2231-7546

The consecutive extraction via different solvents of increasing/decreasing polarity which allowed a pre-fractionation of the extracted compounds is widely used as major method for extraction of polyphenols. Thus the phenolic compounds of Ceratonia siliqua L. at unripe and ripe stages were extracted by four solvents of increasing polarity (hexane chloroform ethyl acetate and acetone/water (70:30 v/v) consecutively. The extracts were evaluated for their total phenolic and flavonoid contents while the antioxidant capacity was assessed in vitro using DPPH radical and hydrogen peroxide (H‚‚ O‚‚ ) scavenging assays. The phenolic profile has been carried out by HPLC-MS/MS. The results showed that the phenolic contents and antioxidant capacity varied with the nature of extracts and the ripening stage. The 70 aqueous acetone and the ethyl acetate extracts contained the highest phenolic and flavonoid contents and subsequently showed a pronounced scavenging activity on DPPH and hydrogen peroxide at unripe and ripe stages. The HPLC-MS/MS allowed the identification of five free phenolic acids (gallic syringic cinnamic p-coumaric and ellagic acid for the first time) and five flavonoids (apigenin naringenin kaempferol quercetin rhamnoside and myricetin rhamnoside) at both ripening stages.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(05)%202017/(28).p...

Abstract

The consecutive extraction via different solvents of increasing/decreasing polarity which allowed a pre-fractionation of the extracted compounds is widely used as major method for extraction of polyphenols. Thus the phenolic compounds of Ceratonia siliqua L. at unripe and ripe stages were extracted by four solvents of increasing polarity (hexane chloroform ethyl acetate and acetone/water (70:30 v/v) consecutively. The extracts were evaluated for their total phenolic and flavonoid contents while the antioxidant capacity was assessed in vitro using DPPH radical and hydrogen peroxide (H‚‚ O‚‚ ) scavenging assays. The phenolic profile has been carried out by HPLC-MS/MS. The results showed that the phenolic contents and antioxidant capacity varied with the nature of extracts and the ripening stage. The 70 aqueous acetone and the ethyl acetate extracts contained the highest phenolic and flavonoid contents and subsequently showed a pronounced scavenging activity on DPPH and hydrogen peroxide at unripe and ripe stages. The HPLC-MS/MS allowed the identification of five free phenolic acids (gallic syringic cinnamic p-coumaric and ellagic acid for the first time) and five flavonoids (apigenin naringenin kaempferol quercetin rhamnoside and myricetin rhamnoside) at both ripening stages.

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Item Type:	Article
AGROVOC Term:	Ceratonia
AGROVOC Term:	Ceratonia siliqua
AGROVOC Term:	Fabaceae
AGROVOC Term:	Carob (tree)
AGROVOC Term:	Bioflavonoids
AGROVOC Term:	Chloroform
AGROVOC Term:	Acetone
AGROVOC Term:	Extraction
AGROVOC Term:	Flavonoids
AGROVOC Term:	Growth inhibitors
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23842

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