Blood glucose reduction of combination of Andrographis paniculata (Burm.f) Ness and Morinda citrifolia L. ethanolic extract in neonatal streptozotocin-induced Type 2 diabetes mellitus rats

Anwar K., . and Wigati D., . and Sudarsono, . and Nugroho A. E., . Blood glucose reduction of combination of Andrographis paniculata (Burm.f) Ness and Morinda citrifolia L. ethanolic extract in neonatal streptozotocin-induced Type 2 diabetes mellitus rats. pp. 2153-2160. ISSN 2231-7546

Andrographis paniculata (Burm. f.) Ness and Morinda citrifolia L. are two medicinal plants that traditionally used by Indonesian people for diabetes treatment. The aim of this study was to evaluate the hypoglycemic effect of A. paniculata ethanolic extract (APEE) and M. citrifolia ethanolic extract (MCEE) combination in neonatal streptozotocin-induced type 2 diabetes mellitus rats in regard to its pancreatic regeneration effect. The powder of dried A. paniculata herbs and M. citrifolia fruits were macerated using 70 ethanol. Phytochemical analysis of andrographolide in APEE and scopoletin in MCEE were performed by TLC-densitometry using stationery phase of silica gel 60 F254 and mobile phase of n-hexane-chloroformmethanol (6:41:3 v/v/v). Hyperglycemic condition in rats was induced with a single dose injection of 90 mg/kg BW streptozotocin (STZ) intraperitoneally in 2 days neonatal rats. Twelve weeks old neonatal STZ-induced rats were orally administrated with several dosage combination of APEE: MCEE (375:125; 250:250; 125:375 mg/kg BW) for 14 consecutive days. Hypoglycemic effect was evaluated by measuring pre-prandial and post-prandial blood glucose levels and other parameters such as pancreatic islet morphology density of pancreatic cells and pancreatic insulin expression. In the study concentration of andrographolide in APEE was 13.72 while scopoletin in MCEE was 0.32. Single treatment of APEE and MCEE exhibited no significant hypoglycemic effect than this of its combination. However the combination of APEE and MCEE exhibited a better improvements in pancreatic islet morphology and increased insulin pancreatic expression than that of single treatment of APEE or MCEE. Combination of APEE: MCEE in dose 250:250 mg/kg BW exhibited better hypoglycemic effect and pancreatic regeneration than other combinations. Combination of A. paniculata and M. citrifolia ethanolic extracts is potential to develop as an anti-diabetic agent.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(05)%202017/(44).p...

Abstract

Andrographis paniculata (Burm. f.) Ness and Morinda citrifolia L. are two medicinal plants that traditionally used by Indonesian people for diabetes treatment. The aim of this study was to evaluate the hypoglycemic effect of A. paniculata ethanolic extract (APEE) and M. citrifolia ethanolic extract (MCEE) combination in neonatal streptozotocin-induced type 2 diabetes mellitus rats in regard to its pancreatic regeneration effect. The powder of dried A. paniculata herbs and M. citrifolia fruits were macerated using 70 ethanol. Phytochemical analysis of andrographolide in APEE and scopoletin in MCEE were performed by TLC-densitometry using stationery phase of silica gel 60 F254 and mobile phase of n-hexane-chloroformmethanol (6:41:3 v/v/v). Hyperglycemic condition in rats was induced with a single dose injection of 90 mg/kg BW streptozotocin (STZ) intraperitoneally in 2 days neonatal rats. Twelve weeks old neonatal STZ-induced rats were orally administrated with several dosage combination of APEE: MCEE (375:125; 250:250; 125:375 mg/kg BW) for 14 consecutive days. Hypoglycemic effect was evaluated by measuring pre-prandial and post-prandial blood glucose levels and other parameters such as pancreatic islet morphology density of pancreatic cells and pancreatic insulin expression. In the study concentration of andrographolide in APEE was 13.72 while scopoletin in MCEE was 0.32. Single treatment of APEE and MCEE exhibited no significant hypoglycemic effect than this of its combination. However the combination of APEE and MCEE exhibited a better improvements in pancreatic islet morphology and increased insulin pancreatic expression than that of single treatment of APEE or MCEE. Combination of APEE: MCEE in dose 250:250 mg/kg BW exhibited better hypoglycemic effect and pancreatic regeneration than other combinations. Combination of A. paniculata and M. citrifolia ethanolic extracts is potential to develop as an anti-diabetic agent.

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Item Type:	Article
AGROVOC Term:	Acanthaceae
AGROVOC Term:	Morinda citrifolia
AGROVOC Term:	Medicinal plants
AGROVOC Term:	Diabetes mellitus
AGROVOC Term:	Animal models
AGROVOC Term:	Insulin
AGROVOC Term:	Experimental design
AGROVOC Term:	Thin layer chromatography
AGROVOC Term:	Quantitative analysis
AGROVOC Term:	Blood glucose
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23857

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