Effect of extraction solvent on total phenolic content total flavonoid content and antioxidant activities of Algerian pomace olive oil


Citation

Ouadah H., . and Lekbir A., . and Khlalfa L., . and Laroui S., . and Ferhat R., . and Alloui-Lombarkia O., . and Kahoul M. A., . Effect of extraction solvent on total phenolic content total flavonoid content and antioxidant activities of Algerian pomace olive oil. pp. 2295-2303. ISSN 2231-7546

Abstract

The present study was aimed to investigate the effect of extraction solvent on oil yield phenolic flavonoid content and antioxidant activities. For this purpose two-phases pomace olive were subjected to solvent extraction using solvents of different polarities (Acetone Hexane methanol Petroleum ether and mixture of Chloroform/Methanol). The phenolic compound in pomace olive oil were extracted by methanol-water. The Folin-Ciocalteu and Aluminium trichloride (AlCl3) method was employed to calculate the total phenolic and flavonoid content respectively while antioxidant capacity was assessed with DPPH -carotene/linoleic acid and FRAP. The oil content of pomace olive obtained after extraction with solvents of different polarities was in the range of 7.55 - 16.92 g/100 g. The best oil yield (16.92) was obtained with the mixture Chloroform/Methanol 25/75. POO8 (extracted with pure Methanol) has the highest content of total phenolic and flavonoid which was 136.78 mg GAE/100g oil and 27.66 mg QE/100g oil respectively. On the other hand POO7 (extracted with mixture Chloroform/ Methanol 25/75) exhibited the highest radical DPPH scavenging power (71.40) and POO5 (extracted with mixture Chloroform/Methanol 75/25) presented the highest bleaching rate of -carotene (83.84). High positive significant correlation (r0.880 p0.01) was found between total phenolic content and FRAP. However no significant correlation was found between total flavonoid content and -carotene bleaching. The results obtained demonstrated that pomace olive oil could have to be used as natural a potential source of bioactive compounds (antioxidants).


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Abstract

The present study was aimed to investigate the effect of extraction solvent on oil yield phenolic flavonoid content and antioxidant activities. For this purpose two-phases pomace olive were subjected to solvent extraction using solvents of different polarities (Acetone Hexane methanol Petroleum ether and mixture of Chloroform/Methanol). The phenolic compound in pomace olive oil were extracted by methanol-water. The Folin-Ciocalteu and Aluminium trichloride (AlCl3) method was employed to calculate the total phenolic and flavonoid content respectively while antioxidant capacity was assessed with DPPH -carotene/linoleic acid and FRAP. The oil content of pomace olive obtained after extraction with solvents of different polarities was in the range of 7.55 - 16.92 g/100 g. The best oil yield (16.92) was obtained with the mixture Chloroform/Methanol 25/75. POO8 (extracted with pure Methanol) has the highest content of total phenolic and flavonoid which was 136.78 mg GAE/100g oil and 27.66 mg QE/100g oil respectively. On the other hand POO7 (extracted with mixture Chloroform/ Methanol 25/75) exhibited the highest radical DPPH scavenging power (71.40) and POO5 (extracted with mixture Chloroform/Methanol 75/25) presented the highest bleaching rate of -carotene (83.84). High positive significant correlation (r0.880 p0.01) was found between total phenolic content and FRAP. However no significant correlation was found between total flavonoid content and -carotene bleaching. The results obtained demonstrated that pomace olive oil could have to be used as natural a potential source of bioactive compounds (antioxidants).

Additional Metadata

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Item Type: Article
AGROVOC Term: Olives
AGROVOC Term: Olive oil
AGROVOC Term: Olea europaea
AGROVOC Term: Aluminium
AGROVOC Term: Drying
AGROVOC Term: Extraction
AGROVOC Term: Solvent extraction
AGROVOC Term: Phenolic compounds
AGROVOC Term: Chloroform
AGROVOC Term: Methanol
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:28
URI: http://webagris.upm.edu.my/id/eprint/23876

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