Efficacy of Thunbergia laurifolia (Rang Jued) aqueous leaf extract for specific biological activities using RAW 264.7 macrophage cells as test model


Citation

Junsi M., . and Siripongvutikorn S., . and Takahashi Yupanqui C., . and Usawakesmanee W., . Efficacy of Thunbergia laurifolia (Rang Jued) aqueous leaf extract for specific biological activities using RAW 264.7 macrophage cells as test model. pp. 2317-2329. ISSN 2231-7546

Abstract

Thunbergia laurifolia or Rang Jued (RJ) has been used in Thailand in folklore medicine since ancient times and has been claimed have anti-inflammatory and antioxidant properties and to be an antidote for several toxins. The phytochemical screening antioxidant and antiinflammatory activities including anti-dote of aqueous leaf extract on RAW 264.7 macrophage cells were evaluated. Data indicated that the extract contained phenols flavonoids tannins sterols and cardiac-glycoside groups. Total extractable phenolic contents (TPC) and antioxidant activities of the extract were quite high level compared with other researches data. The high performance liquid chromatographic (HPLC) analysis of phenolics and flavonoids indicated the presence of pyrogallol caffeic acid rosmarinic acid and rutin compounds. The extract could not only reduce nitric oxide production but also increased cell proliferation and was judged as cytotoxicity even in low level. In addition the extract could be used as preventing agent not for curing treatment for RAW 264.7 macrophage cells treated with cadmium (Cd) and chlorpyrifos (CP). However preventing efficacy of the extract to CP toxicity was higher compared with Cd.


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Abstract

Thunbergia laurifolia or Rang Jued (RJ) has been used in Thailand in folklore medicine since ancient times and has been claimed have anti-inflammatory and antioxidant properties and to be an antidote for several toxins. The phytochemical screening antioxidant and antiinflammatory activities including anti-dote of aqueous leaf extract on RAW 264.7 macrophage cells were evaluated. Data indicated that the extract contained phenols flavonoids tannins sterols and cardiac-glycoside groups. Total extractable phenolic contents (TPC) and antioxidant activities of the extract were quite high level compared with other researches data. The high performance liquid chromatographic (HPLC) analysis of phenolics and flavonoids indicated the presence of pyrogallol caffeic acid rosmarinic acid and rutin compounds. The extract could not only reduce nitric oxide production but also increased cell proliferation and was judged as cytotoxicity even in low level. In addition the extract could be used as preventing agent not for curing treatment for RAW 264.7 macrophage cells treated with cadmium (Cd) and chlorpyrifos (CP). However preventing efficacy of the extract to CP toxicity was higher compared with Cd.

Additional Metadata

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Item Type: Article
AGROVOC Term: Acanthaceae
AGROVOC Term: Bioflavonoids
AGROVOC Term: Macrophages
AGROVOC Term: Cadmium
AGROVOC Term: Caffeic acid
AGROVOC Term: Chlorpyrifos
AGROVOC Term: Antioxidants
AGROVOC Term: Inflammation
AGROVOC Term: Screening tests
AGROVOC Term: Extracts
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:28
URI: http://webagris.upm.edu.my/id/eprint/23879

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