Peptides obtained from proteins of cobia (Rachycentron canadum): A study of potentially safe antioxidants for food

Fonseca R. A. S., . and Silva C. M., . and Rosa G. F., . and Prentice C., . Peptides obtained from proteins of cobia (Rachycentron canadum): A study of potentially safe antioxidants for food. pp. 2500-2508. ISSN 2231-7546

The fish and its by-products from industrial processing are a rich source of protein. For this reason cobia (Rachycentron canadum) a large and easily adaptable to aquaculture species has emerged as an option for obtaining peptides. Enzymatic proteolysis is able to release inactive biopeptides “ which may have greater activity when separated into their peptide fractions “ from intact proteins. However as they can be applied in food they should be evaluated for any possible harm to health. Thus this study aimed to evaluate the ability of the protein hydrolysates of cobia (muscle and waste) obtained by the action of Alcalase Flavourzyme and Protamex enzymes (whole and their fractions less and major than 3 kDa) to inhibit the oxidation of meat food and ensure their food security. The hydrolysates studied in this work demonstrated antioxidant activity through chemical methods in vitro. All hydrolysates and their fractions were not cytotoxic to zebrafish (Danio rerio) hepatocytes at the concentrations of 0.1 1 10 and 100 g/mL at 0 24 48 and 72 h. By determining the inhibition of thiobarbituric acid reactive substances in bacon and ground beef it was found that the majority of the hydrolysates presented this capacity highlighting the peptide fractions major than 3 kDa of muscle hydrolysates which reduced by around 50 the TBARS (thiobarbituric acid reactive substances) content formed in the ground beef and by more than 80 in bacon probably because the bacon has a higher content of lipid. The enzyme Protamex provided hydrolyzed with more antioxidant activity. Therefore these results indicate that the hydrolysates studied have the potential to be safe physicochemically not cytotoxic used in foods as antioxidants.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(06)%202017/(31).p...

Abstract

The fish and its by-products from industrial processing are a rich source of protein. For this reason cobia (Rachycentron canadum) a large and easily adaptable to aquaculture species has emerged as an option for obtaining peptides. Enzymatic proteolysis is able to release inactive biopeptides “ which may have greater activity when separated into their peptide fractions “ from intact proteins. However as they can be applied in food they should be evaluated for any possible harm to health. Thus this study aimed to evaluate the ability of the protein hydrolysates of cobia (muscle and waste) obtained by the action of Alcalase Flavourzyme and Protamex enzymes (whole and their fractions less and major than 3 kDa) to inhibit the oxidation of meat food and ensure their food security. The hydrolysates studied in this work demonstrated antioxidant activity through chemical methods in vitro. All hydrolysates and their fractions were not cytotoxic to zebrafish (Danio rerio) hepatocytes at the concentrations of 0.1 1 10 and 100 g/mL at 0 24 48 and 72 h. By determining the inhibition of thiobarbituric acid reactive substances in bacon and ground beef it was found that the majority of the hydrolysates presented this capacity highlighting the peptide fractions major than 3 kDa of muscle hydrolysates which reduced by around 50 the TBARS (thiobarbituric acid reactive substances) content formed in the ground beef and by more than 80 in bacon probably because the bacon has a higher content of lipid. The enzyme Protamex provided hydrolyzed with more antioxidant activity. Therefore these results indicate that the hydrolysates studied have the potential to be safe physicochemically not cytotoxic used in foods as antioxidants.

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Item Type:	Article
AGROVOC Term:	Rachycentron canadum
AGROVOC Term:	Peptides
AGROVOC Term:	Proteins
AGROVOC Term:	Food security
AGROVOC Term:	Food industry
AGROVOC Term:	Lipid peroxidation
AGROVOC Term:	Enzymes
AGROVOC Term:	Protein hydrolysate
AGROVOC Term:	Enzymatic hydrolysis
AGROVOC Term:	Ultrafiltration
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23913

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