Microwave-assisted extraction of bioactive compounds from blueberry (Vaccinium ashei Reade) and their antioxidant and antimicrobial capacity


Citation

Mello R. de O., . and Viera V. B., . and Vaucher R. de A., . and Dressler V. L., . and Piovesan N., . and Bizzi C. A., . and Santos R.C. V. dos., . and Fries L. L. M., . Microwave-assisted extraction of bioactive compounds from blueberry (Vaccinium ashei Reade) and their antioxidant and antimicrobial capacity. pp. 2526-2533. ISSN 2231-7546

Abstract

The aim of this study was to evaluate the content of phenolic compounds flavonoids and total anthocyanins as well as the antioxidant and antimicrobial activity of blueberry extracts (Vaccinium ashei Reade) of the Climax variety. The extracts were obtained by focused microwave extraction at different temperatures (30 40 50 and 60C) and solvent concentrations (60 and 80 hydroethanolic). The antioxidant activity of the extracts was quantified by the DPPH radical sequestration method inhibition of auto-oxidation of the -carotene system ABTS FRAP and IC50. The antimicrobial activity was evaluated by the disk diffusion method. The antioxidant activity (FRAP ABTS and DPPH) total phenolics and total anthocyanins showed a positive linear trend in relation to the extraction temperature with no significant difference between the solvent concentrations (60 and 80). The IC50 and -carotene showed the greatest inhibition at 60C at solvent concentrations of 60 and 80 respectively. The flavonoids showed no significant difference in terms of the extraction temperature or the concentration of solvent that was used. No antimicrobial activity was detected in the extracts in relation to the tested microorganisms. The results showed a positive correlation between the total phenolic content and the determined antioxidant activities. This study confirms the potential of this fruit as a source of phenolic compounds with high antioxidant activity and it also provides a new and efficient technique to extract bioactive compounds using microwaves.


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Abstract

The aim of this study was to evaluate the content of phenolic compounds flavonoids and total anthocyanins as well as the antioxidant and antimicrobial activity of blueberry extracts (Vaccinium ashei Reade) of the Climax variety. The extracts were obtained by focused microwave extraction at different temperatures (30 40 50 and 60C) and solvent concentrations (60 and 80 hydroethanolic). The antioxidant activity of the extracts was quantified by the DPPH radical sequestration method inhibition of auto-oxidation of the -carotene system ABTS FRAP and IC50. The antimicrobial activity was evaluated by the disk diffusion method. The antioxidant activity (FRAP ABTS and DPPH) total phenolics and total anthocyanins showed a positive linear trend in relation to the extraction temperature with no significant difference between the solvent concentrations (60 and 80). The IC50 and -carotene showed the greatest inhibition at 60C at solvent concentrations of 60 and 80 respectively. The flavonoids showed no significant difference in terms of the extraction temperature or the concentration of solvent that was used. No antimicrobial activity was detected in the extracts in relation to the tested microorganisms. The results showed a positive correlation between the total phenolic content and the determined antioxidant activities. This study confirms the potential of this fruit as a source of phenolic compounds with high antioxidant activity and it also provides a new and efficient technique to extract bioactive compounds using microwaves.

Additional Metadata

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Item Type: Article
AGROVOC Term: Vaccinium
AGROVOC Term: Vaccinium ashei
AGROVOC Term: Blueberries
AGROVOC Term: Antimicrobial properties
AGROVOC Term: Antioxidants
AGROVOC Term: Chemical composition
AGROVOC Term: Evaluation
AGROVOC Term: Extraction
AGROVOC Term: Heating
AGROVOC Term: Microwave treatment
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:28
URI: http://webagris.upm.edu.my/id/eprint/23916

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