Tocotrienols reduce monocytes adhesion to stimulated human endothelial cells

Abdul Manaf Ali, . and Froemming Gabriele R .A., . and Hapizah Md Nawawi, . and Suhaila Abd Muid, . and Thuhairah Hasrah Abdul Rahman, . Tocotrienols reduce monocytes adhesion to stimulated human endothelial cells. pp. 2580-2585. ISSN 2231-7546

The anti-atherosclerotics activity of tocotrienols (TCT) compared to alpha-Tocopherol (-TOC) in vitro study is not much being reported especially in human endothelial cells. The aim of the present study was to study the effects of TTMF TCT and -TOC on monocytes adherence to stimulated endothelial cells and to investigate the correlation between monocytes adherence and adhesion molecules in endothelial cells treated with TTMF pure TCT isomers and -TOC. Human umbilical vein endothelial cells (HUVECs) were incubated with TTMF TCT isomers and -TOC (0.3-10 M) together with lipopolysaccharide LPS (1 g/ml). Monocytes adherence was measured by Rose Bengal staining. Soluble ICAM-1 VCAM-1 and e-selectin and NFB binding were measured by ELISA. TTMF and TCT isomers inhibit monocytes adhesion to LPS-stimulated HUVECs but not -TOC. ´-TCT exhibit the highest inhibition of monocytes adhesion compared to the other TCT isomers. Only TCT isomers show positive correlation of monocytes adhesion with certain adhesion molecules and NFB but not TTMF and -TOC. In conclusion TTMF and TCT isomers exhibit reduction of adhesion of monocytes to LPS stimulated endothelial cells. The reduction of monocytes adhesion by TCT isomers especially ´-TCT are positively correlated with reduction of adhesion molecules and NFB deactivation. It can be suggested that TCT especially the ´-TCT isomers is beneficial in the prevention of early atherogenesis in human.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(06)%202017/(41).p...

Abstract

The anti-atherosclerotics activity of tocotrienols (TCT) compared to alpha-Tocopherol (-TOC) in vitro study is not much being reported especially in human endothelial cells. The aim of the present study was to study the effects of TTMF TCT and -TOC on monocytes adherence to stimulated endothelial cells and to investigate the correlation between monocytes adherence and adhesion molecules in endothelial cells treated with TTMF pure TCT isomers and -TOC. Human umbilical vein endothelial cells (HUVECs) were incubated with TTMF TCT isomers and -TOC (0.3-10 M) together with lipopolysaccharide LPS (1 g/ml). Monocytes adherence was measured by Rose Bengal staining. Soluble ICAM-1 VCAM-1 and e-selectin and NFB binding were measured by ELISA. TTMF and TCT isomers inhibit monocytes adhesion to LPS-stimulated HUVECs but not -TOC. ´-TCT exhibit the highest inhibition of monocytes adhesion compared to the other TCT isomers. Only TCT isomers show positive correlation of monocytes adhesion with certain adhesion molecules and NFB but not TTMF and -TOC. In conclusion TTMF and TCT isomers exhibit reduction of adhesion of monocytes to LPS stimulated endothelial cells. The reduction of monocytes adhesion by TCT isomers especially ´-TCT are positively correlated with reduction of adhesion molecules and NFB deactivation. It can be suggested that TCT especially the ´-TCT isomers is beneficial in the prevention of early atherogenesis in human.

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Item Type:	Article
AGROVOC Term:	Tocotrienols
AGROVOC Term:	Tocopherols
AGROVOC Term:	Dyes
AGROVOC Term:	In vitro
AGROVOC Term:	Inhibition
AGROVOC Term:	Cell culture
AGROVOC Term:	Cytotoxicity
AGROVOC Term:	Lipopolysaccharides
AGROVOC Term:	Enzyme linked immunosorbent assay
AGROVOC Term:	Monocytes
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23923

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