The efficiency and the correlation between testing methods on antimicrobial and antioxidant activities of selected medicinal essential oils

Utakod N., . and Laosripaiboon W., . and Chunhachart O., . and Issakul K., . The efficiency and the correlation between testing methods on antimicrobial and antioxidant activities of selected medicinal essential oils. pp. 2616-2624. ISSN 2231-7546

Eight selected medicinal essential oils”calamus clove betel lime Chinese cinnamon lemon grass vetiver and pine were determined for their antimicrobial and antioxidant activities. The inhibitory effect against pathogenic microorganisms using agar disc and agar well-diffusion methods demonstrated that Candida albican was extremely sensitive to Chinese cinnamon oil with an inhibition zone diameter of 2.4 and 1.5 cm for agar disc and agar well-diffusion tests respectively. This microbe was also the most susceptible with MIC and MFC values of 0.075 L. mL-. The results from the MIC test showed that Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) were more resistant to Chinese cinnamon oil than Grampositive bacteria (Staphylococcus aureus and Staphylococcus epidermidis). The antioxidant activity tested using DPPH ABTS and FRAP methods revealed that clove and betel oils were the strongest antioxidant agents with non-significant differences in comparison to the control. According to the Pearsons correlation coefficient for the antimicrobial test C. albicans had the highest significant correlation between the agar disc and agar well-diffusion methods (r 0.927). Gram-negative bacteria also demonstrated a stronger and significant correlation than Gram-positive bacteria. The antioxidant testing methods indicated that DPPH ABTS and FRAP showed strong positive correlations with each other with coefficients ranging from 0.808 to 0.989. In contrast H‚‚ O‚‚ scavenging assay showed no correlations. These results suggested that Chinese cinnamon and clove oils are the most effective natural agents for antimicrobial and antioxidant activities respectively. Moreover a positive correlation was found between the testing methods in both antimicrobial and antioxidant activity tests.

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Abstract

Eight selected medicinal essential oils”calamus clove betel lime Chinese cinnamon lemon grass vetiver and pine were determined for their antimicrobial and antioxidant activities. The inhibitory effect against pathogenic microorganisms using agar disc and agar well-diffusion methods demonstrated that Candida albican was extremely sensitive to Chinese cinnamon oil with an inhibition zone diameter of 2.4 and 1.5 cm for agar disc and agar well-diffusion tests respectively. This microbe was also the most susceptible with MIC and MFC values of 0.075 L. mL-. The results from the MIC test showed that Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) were more resistant to Chinese cinnamon oil than Grampositive bacteria (Staphylococcus aureus and Staphylococcus epidermidis). The antioxidant activity tested using DPPH ABTS and FRAP methods revealed that clove and betel oils were the strongest antioxidant agents with non-significant differences in comparison to the control. According to the Pearsons correlation coefficient for the antimicrobial test C. albicans had the highest significant correlation between the agar disc and agar well-diffusion methods (r 0.927). Gram-negative bacteria also demonstrated a stronger and significant correlation than Gram-positive bacteria. The antioxidant testing methods indicated that DPPH ABTS and FRAP showed strong positive correlations with each other with coefficients ranging from 0.808 to 0.989. In contrast H‚‚ O‚‚ scavenging assay showed no correlations. These results suggested that Chinese cinnamon and clove oils are the most effective natural agents for antimicrobial and antioxidant activities respectively. Moreover a positive correlation was found between the testing methods in both antimicrobial and antioxidant activity tests.

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Item Type:	Article
AGROVOC Term:	Medicinal plants
AGROVOC Term:	Acorus calamus
AGROVOC Term:	Eugenia caryophyllata
AGROVOC Term:	Piper betle
AGROVOC Term:	Citrus aurantiifolia
AGROVOC Term:	Cinnamomum cassia
AGROVOC Term:	Cymbopogon citratus
AGROVOC Term:	Vetiveria zizanioides
AGROVOC Term:	Pinus sylvestris
AGROVOC Term:	Essential oils
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23928

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