The potential of Lactobacillus rhamnosus and Lactobacillus plantarum isolated from goats milk in inhibiting Salmonella typhimurium ATCC 14028 infections in rats


Citation

Setyawardani T., . and Rahayu W. P., . and Palupi N. S., . and Sumarmono J., . The potential of Lactobacillus rhamnosus and Lactobacillus plantarum isolated from goats milk in inhibiting Salmonella typhimurium ATCC 14028 infections in rats. pp. 2625-2631. ISSN 2231-7546

Abstract

Probiotic is live non-pathogenic microorganisms that give beneficial effects on health when they are administered in adequate amounts. The objective of the study was to evaluate the influence of Lactic Acid Bacteria (LAB) isolates (L. rhamnosus and L. plantarum) as well as cheese containing the probiotics on microflora profiles morphological profile of ileum and caecum and immunomodulator potency by measuring lymphocyte proliferation and IgA levels in rats. Male Sprague Dawley rats were fed with the probiotics or cheese containing the probiotics for 10 days infected with S. typhimurium for 3 days and continued to be fed with or without the probiotics or the cheese. A total of 6 treatments were applied which were: (protyp-pro pro-typ-std che-typ-che che-typ-std pro-PBS-pro and std-typ-std). The measured variables were the number of LAB and S. typhimurium colonies lymphocyte cells and the level of SIgA. The results showed that the highest number of LAB in the ileum and caecum in probiotic fed rats (pro-typ-std followed by pro-typ-pro) as compared to the control whereas number of S. typhimurium was lower. The study showed that the treatment of probiotic isolate was able to improve the number of lymphocyte during the first 10 days during the infection of S. typhimurium and post infection stage. The treatment of probiotic isolate was able to improve SIgA at the time of S. typhimurium intervention. In conclusion mixed isolates of L. rhamnosus and L. plantarum and cheese containing the probiotics were able to show preventive and remedial functions during S. typhimurium ATCC 14028 infection thus demonstrate the potential to be used as probiotic cultures.


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Abstract

Probiotic is live non-pathogenic microorganisms that give beneficial effects on health when they are administered in adequate amounts. The objective of the study was to evaluate the influence of Lactic Acid Bacteria (LAB) isolates (L. rhamnosus and L. plantarum) as well as cheese containing the probiotics on microflora profiles morphological profile of ileum and caecum and immunomodulator potency by measuring lymphocyte proliferation and IgA levels in rats. Male Sprague Dawley rats were fed with the probiotics or cheese containing the probiotics for 10 days infected with S. typhimurium for 3 days and continued to be fed with or without the probiotics or the cheese. A total of 6 treatments were applied which were: (protyp-pro pro-typ-std che-typ-che che-typ-std pro-PBS-pro and std-typ-std). The measured variables were the number of LAB and S. typhimurium colonies lymphocyte cells and the level of SIgA. The results showed that the highest number of LAB in the ileum and caecum in probiotic fed rats (pro-typ-std followed by pro-typ-pro) as compared to the control whereas number of S. typhimurium was lower. The study showed that the treatment of probiotic isolate was able to improve the number of lymphocyte during the first 10 days during the infection of S. typhimurium and post infection stage. The treatment of probiotic isolate was able to improve SIgA at the time of S. typhimurium intervention. In conclusion mixed isolates of L. rhamnosus and L. plantarum and cheese containing the probiotics were able to show preventive and remedial functions during S. typhimurium ATCC 14028 infection thus demonstrate the potential to be used as probiotic cultures.

Additional Metadata

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Item Type: Article
AGROVOC Term: Lactobacillus
AGROVOC Term: Lactobacillus plantarum
AGROVOC Term: Salmonella typhimurium
AGROVOC Term: Lactic acid bacteria
AGROVOC Term: Lactic acid
AGROVOC Term: Goats
AGROVOC Term: Rats
AGROVOC Term: Animal models
AGROVOC Term: Cheese
AGROVOC Term: Infection
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:28
URI: http://webagris.upm.edu.my/id/eprint/23929

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