Culture dependent and independent studies on emerging food-borne pathogens Cronobacter sakazakii Klebsiella pneumoniae and Enterococcus faecalis in Indian food

Gautam R. K., . and Kakatkar A. S., . and Pansare Godambe L., . and Shashidhar R., . Culture dependent and independent studies on emerging food-borne pathogens Cronobacter sakazakii Klebsiella pneumoniae and Enterococcus faecalis in Indian food. pp. 2645-2651. ISSN 2231-7546

Emerging food-borne pathogens Cronobacter sakazakii Klebsiella pneumoniae and Enterococcus faecalis may cause food-borne diseases in neonates elderly and immunocompromised patients. Data on prevalence of emerging pathogens in food from India is lacking. Sixty food samples were analyzed from local markets in Mumbai for the presence of C. sakazakii K. Pneumoniae and E. faecalis. Present study shows that incidence of C. sakazakii and K. pneumoniae was highest in sprouts and least in milk (Table 1). Total 35 and 85 of food samples were positive for C. sakazakii and K. pneumoniae respectively. Culture independent species specific PCR based method was better than conventional culture based method in detecting these pathogens. E. faecalis was not detected in any of the food samples. All the isolates of C. sakazakii and K. pneumoniae were resistant to ampicillin. C. sakazakii was also resistant to cephalothin and K. pneumoniae was resistant to many other beta-lactam antibiotics like amikacin aztreonam and amoxicillin-clavulanic acid. Our results suggest that conventional method should be carried out in combination with molecular methods for the accurate identification of these pathogens. Increase in drug resistance amongst emerging pathogens is also a concern.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(06)%202017/(50).p...

Abstract

Emerging food-borne pathogens Cronobacter sakazakii Klebsiella pneumoniae and Enterococcus faecalis may cause food-borne diseases in neonates elderly and immunocompromised patients. Data on prevalence of emerging pathogens in food from India is lacking. Sixty food samples were analyzed from local markets in Mumbai for the presence of C. sakazakii K. Pneumoniae and E. faecalis. Present study shows that incidence of C. sakazakii and K. pneumoniae was highest in sprouts and least in milk (Table 1). Total 35 and 85 of food samples were positive for C. sakazakii and K. pneumoniae respectively. Culture independent species specific PCR based method was better than conventional culture based method in detecting these pathogens. E. faecalis was not detected in any of the food samples. All the isolates of C. sakazakii and K. pneumoniae were resistant to ampicillin. C. sakazakii was also resistant to cephalothin and K. pneumoniae was resistant to many other beta-lactam antibiotics like amikacin aztreonam and amoxicillin-clavulanic acid. Our results suggest that conventional method should be carried out in combination with molecular methods for the accurate identification of these pathogens. Increase in drug resistance amongst emerging pathogens is also a concern.

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Item Type:	Article
AGROVOC Term:	Klebsiella pneumoniae
AGROVOC Term:	Enterococcus faecalis
AGROVOC Term:	Foodborne diseases
AGROVOC Term:	Human diseases
AGROVOC Term:	Disease prevalence
AGROVOC Term:	Ampicillin
AGROVOC Term:	Antibiotics
AGROVOC Term:	Isolation
AGROVOC Term:	Methodology
AGROVOC Term:	PCR
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23932

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