Kinetic performance of Cross-Linked Enzyme Aggregates (CLEA)-amylase from Zophobas morio

Muhammad Noor Easa, . and Faridah Yusof, . and Amanatuzzakiah Abd Halim, . Kinetic performance of Cross-Linked Enzyme Aggregates (CLEA)-amylase from Zophobas morio. S320-S324. ISSN 2231-7546

Many studies have been done on various species of insects to investigate their potential use in industries. This is because insects have high protein content which could be further manipulated. Due to its eating habit Zophobas morio larvae also known as super mealworm has been shown to have high amylase activity. In this study amylase from super mealworm has been immobilized via Cross-Linked Enzyme Aggregates (CLEA) technique and its kinetic performance evaluated. CLEA is one of the best immobilization method with respect to enzyme stability and reusability. Kinetic performance of both free and CLEA-amylase were evaluated based on the Michaelis-Menten model. Results obtained based on Hanes-Woolf LineweaverBurk Eadie-Hofstee and Hyperbolic Regression plots showed that the kinetic parameters Vmax and KM changed upon immobilization. For CLEA-amylase Hanes-Woolf plot showed the bestfitted model based on R with Vmax 1.068 mM/min and KM 0.182 mM however LineweaverBurk plot was used to obtain the kinetic parameters for free amylase with Vmax and KM of 17.230 mM/min and 2.470 mM respectively. Thus it is observed that upon immobilization Vmax for amylase dropped appreciably however much lower substrate concentration is needed to saturate the enzymatic sites to reach its maximum catalytic efficiency. The result from this study might open the new path in discovering the potential use of insects in industrial applications for example making use of the recovered enzymes in the detergent industry.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(07)%202017%20supp...

Abstract

Many studies have been done on various species of insects to investigate their potential use in industries. This is because insects have high protein content which could be further manipulated. Due to its eating habit Zophobas morio larvae also known as super mealworm has been shown to have high amylase activity. In this study amylase from super mealworm has been immobilized via Cross-Linked Enzyme Aggregates (CLEA) technique and its kinetic performance evaluated. CLEA is one of the best immobilization method with respect to enzyme stability and reusability. Kinetic performance of both free and CLEA-amylase were evaluated based on the Michaelis-Menten model. Results obtained based on Hanes-Woolf LineweaverBurk Eadie-Hofstee and Hyperbolic Regression plots showed that the kinetic parameters Vmax and KM changed upon immobilization. For CLEA-amylase Hanes-Woolf plot showed the bestfitted model based on R with Vmax 1.068 mM/min and KM 0.182 mM however LineweaverBurk plot was used to obtain the kinetic parameters for free amylase with Vmax and KM of 17.230 mM/min and 2.470 mM respectively. Thus it is observed that upon immobilization Vmax for amylase dropped appreciably however much lower substrate concentration is needed to saturate the enzymatic sites to reach its maximum catalytic efficiency. The result from this study might open the new path in discovering the potential use of insects in industrial applications for example making use of the recovered enzymes in the detergent industry.

[error in script]

Item Type:	Article
AGROVOC Term:	Tenebrionidae
AGROVOC Term:	Insects
AGROVOC Term:	Amylases
AGROVOC Term:	Enzymes
AGROVOC Term:	Evaluation
AGROVOC Term:	Extraction
AGROVOC Term:	Stirring
AGROVOC Term:	Immobilization
AGROVOC Term:	Protein content
AGROVOC Term:	Research
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 05:34
URI:	http://webagris.upm.edu.my/id/eprint/23949

Actions (login required)

View Item