Effects of Sargassum oligocystum and Padina australis extract on adipogenesis and adipolysis in 3T3-L1 cells

Bakhtiar M. T., . and Hasna Ahmad, . and Octavianti F., . and Jaswir I., . and Susanti D., . Effects of Sargassum oligocystum and Padina australis extract on adipogenesis and adipolysis in 3T3-L1 cells. S395-S401. ISSN 2231-7546

In recent years the search for natural safe and effective therapies for the management of obesity has become important. The present study investigated the potential of brown seaweeds Sargassum oligocystum and Padina australis from Malaysian waters as natural alternatives for the management of obesity. Both seaweeds were macerated using acetone at room temperature for ten hours. The S. oligocystum extracts (SE) and P. australis extracts (PE) were then applied to 3T3-L1 cells during the differentiation stage and during the mature stage of the adipocyte life cycle to assess the effects of extracts on adipogenesis and adipolysis. Application of SE at 12.5 and 50 g/ml to the cells decreased adipogenesis by 71.7 and 84.8 respectively while cells treated with 12.5 and 50 g/ml PE showed 85.7 and 89.0 adipogenesis respectively compared to control. Application of SE and PE to mature lipid cells stimulated adipolysis and the release of glycerol into the culture media. Application of SE at 12.5 and 50 g g/ ml in the cell media induced glycerol release by up to 88.6 and 93.0 respectively while PE increased glycerol release up to 92.9 and 95.6 respectively compared to isoproterenol. This study demonstrates the potential of whole brown seaweed extracts from S. oligocystum and P. australis collected from Malaysian waters as natural anti- obesity agents. Incorporation of the brown seaweed into the diet as a functional food will be useful for prevention and treatment of obesity.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(07)%202017%20supp...

Abstract

In recent years the search for natural safe and effective therapies for the management of obesity has become important. The present study investigated the potential of brown seaweeds Sargassum oligocystum and Padina australis from Malaysian waters as natural alternatives for the management of obesity. Both seaweeds were macerated using acetone at room temperature for ten hours. The S. oligocystum extracts (SE) and P. australis extracts (PE) were then applied to 3T3-L1 cells during the differentiation stage and during the mature stage of the adipocyte life cycle to assess the effects of extracts on adipogenesis and adipolysis. Application of SE at 12.5 and 50 g/ml to the cells decreased adipogenesis by 71.7 and 84.8 respectively while cells treated with 12.5 and 50 g/ml PE showed 85.7 and 89.0 adipogenesis respectively compared to control. Application of SE and PE to mature lipid cells stimulated adipolysis and the release of glycerol into the culture media. Application of SE at 12.5 and 50 g g/ ml in the cell media induced glycerol release by up to 88.6 and 93.0 respectively while PE increased glycerol release up to 92.9 and 95.6 respectively compared to isoproterenol. This study demonstrates the potential of whole brown seaweed extracts from S. oligocystum and P. australis collected from Malaysian waters as natural anti- obesity agents. Incorporation of the brown seaweed into the diet as a functional food will be useful for prevention and treatment of obesity.

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Item Type:	Article
AGROVOC Term:	Sargassum oligocystum
AGROVOC Term:	Padina
AGROVOC Term:	Brown seaweeds
AGROVOC Term:	Aquatic plants
AGROVOC Term:	Obesity
AGROVOC Term:	Extraction
AGROVOC Term:	Cell culture
AGROVOC Term:	Culture media
AGROVOC Term:	Cell differentiation
AGROVOC Term:	Life cycle
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 05:34
URI:	http://webagris.upm.edu.my/id/eprint/23957

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