Influence on cultivation conditions in the heterotrophic lipid production of the microalga Chlorella minutissima


Citation

Ferreira S. P., . and Holz J. C. P., . and Costa J. A. V., . Influence on cultivation conditions in the heterotrophic lipid production of the microalga Chlorella minutissima. pp. 408-417. ISSN 2231-7546

Abstract

Chlorella among microalgae has possibly been the most used for feeding as well as the most researched in heterotrophic growing lately due to the highest lipid yield. The objective of this work was to study the effect of carbon and nitrogen concentration for lipid productivity and biomass production of microalga Chlorella minutissima cultivated in absence of light. The complete factorial design used was type 22 with three repetitions at the central point having as glucose concentration factor (1 5 e 9 g.L-1) and as sodium nitrate (NaNO3) (0.2; 0.75 and 1.3 g.L-1) and total lipid and biomass concentration as responses with a total number of 7 experiments. The cultivation carried out with 9 g.L-1 of glucose and 0.2 g.L-1 of NaNO3 was the one that presented better lipid concentration (29.38 w/w) better cell growth (0.89 g.L-1) and a lipid profile comprising saturated monounsaturated and polyunsaturated fatty acids in 26.6 w/w 62.4 w/w and 11 w/w concentrations respectively.


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Abstract

Chlorella among microalgae has possibly been the most used for feeding as well as the most researched in heterotrophic growing lately due to the highest lipid yield. The objective of this work was to study the effect of carbon and nitrogen concentration for lipid productivity and biomass production of microalga Chlorella minutissima cultivated in absence of light. The complete factorial design used was type 22 with three repetitions at the central point having as glucose concentration factor (1 5 e 9 g.L-1) and as sodium nitrate (NaNO3) (0.2; 0.75 and 1.3 g.L-1) and total lipid and biomass concentration as responses with a total number of 7 experiments. The cultivation carried out with 9 g.L-1 of glucose and 0.2 g.L-1 of NaNO3 was the one that presented better lipid concentration (29.38 w/w) better cell growth (0.89 g.L-1) and a lipid profile comprising saturated monounsaturated and polyunsaturated fatty acids in 26.6 w/w 62.4 w/w and 11 w/w concentrations respectively.

Additional Metadata

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Item Type: Article
AGROVOC Term: Chlorella
AGROVOC Term: Green algae
AGROVOC Term: Culture of microorganisms
AGROVOC Term: Cultivation
AGROVOC Term: Carbon
AGROVOC Term: Nitrogen compounds
AGROVOC Term: Glucose
AGROVOC Term: Fatty acids
AGROVOC Term: Biomass
AGROVOC Term: Polyunsaturated fatty acids
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:28
URI: http://webagris.upm.edu.my/id/eprint/24032

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