Surface morphological changes of pathogenic Acanthamoeba spp. treated with Mahanimbine

Fatimah H., . and Nakisah M. A., . and A. M. Ali, . and S. Aspollah, . Surface morphological changes of pathogenic Acanthamoeba spp. treated with Mahanimbine. pp. 67-71. ISSN 2180-1983

Acanthamoeba spp. are the most common free-living amoebae (FLA) found abundantly in the environment. These opportunistic organisms are causative agents of Acanthamoeba keratitis a painful eye infection that could result in loss of vision and is commonly associated with users of contact lenses. Two species of Acanthamoeba namely Acanthamoeba sp. (SW isolate) and A. polyphaga (CCAP 1501/3A) were chosen in order to study the mechanism of action of a plant compound mahanimbine against these amoebae. Scanning electron microscopy (SEM) was used to analyze the cellular morphological changes that occurred to the amoebae after treatment with this pure compound at their IC50 concentration. The IC50 value for Acanthamoeba sp. (SW isolate) and A. polyphaga (CCAP 1501/3A) were 7.2 g/mL and 1.00 g/mL respectively. The treatment was carried out for 72 hr at 30 C and involved culturing the amoebae on cover slips in six-well culture plates followed by normal processing methods of the amoebae for SEM. The evidences of damage on the trophozoites of amoebae were their rounded forms with cystic appearances and reduction in size. The number of acanthapodia decreased as compared to numerous spine-like acanthapodia as observed on untreated trophozoites. The research findings in the current study revealed that mahanimbine can cause cellular morphological changes especially to the cell shape and number of acanthapodia on the amoeba trophozoites.

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Abstract

Acanthamoeba spp. are the most common free-living amoebae (FLA) found abundantly in the environment. These opportunistic organisms are causative agents of Acanthamoeba keratitis a painful eye infection that could result in loss of vision and is commonly associated with users of contact lenses. Two species of Acanthamoeba namely Acanthamoeba sp. (SW isolate) and A. polyphaga (CCAP 1501/3A) were chosen in order to study the mechanism of action of a plant compound mahanimbine against these amoebae. Scanning electron microscopy (SEM) was used to analyze the cellular morphological changes that occurred to the amoebae after treatment with this pure compound at their IC50 concentration. The IC50 value for Acanthamoeba sp. (SW isolate) and A. polyphaga (CCAP 1501/3A) were 7.2 g/mL and 1.00 g/mL respectively. The treatment was carried out for 72 hr at 30 C and involved culturing the amoebae on cover slips in six-well culture plates followed by normal processing methods of the amoebae for SEM. The evidences of damage on the trophozoites of amoebae were their rounded forms with cystic appearances and reduction in size. The number of acanthapodia decreased as compared to numerous spine-like acanthapodia as observed on untreated trophozoites. The research findings in the current study revealed that mahanimbine can cause cellular morphological changes especially to the cell shape and number of acanthapodia on the amoeba trophozoites.

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Item Type:	Article
AGROVOC Term:	Acanthamoeba
AGROVOC Term:	Amoeba
AGROVOC Term:	Pathogenicity
AGROVOC Term:	Eye diseases
AGROVOC Term:	Keratitis
AGROVOC Term:	Analytical methods
AGROVOC Term:	Scanning microscopy
AGROVOC Term:	Cell structure
AGROVOC Term:	morphology
AGROVOC Term:	Cell membranes
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/24108

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