Citation
Nordiana Hanim Mohd Nor, . and Singh Rajinder, . and Smith S. W., . and Schwarzacher Trude, . and Noorhariza Mohd Zaki, . and Madon M., . and Muhammad Azwan Zulkifli, . and Heslop-Harrison J. S., . Short communication: towards development of Elaeis guineensis chromosome-arm specific markers and their utility across the Elaeis genus. pp. 594-599. ISSN 1511-2780
Abstract
The development of Elaeis guineensis chromosome-arm specific markers is required for linking the genetic sequence and chromosomal maps valuable for comparative studies hybridisation and breeding. Here we aimed to develop strategies exploiting assembled short shotgun-sequence reads to identify markers for in situ hybridisation to identify arms and large-scale organisation of pseudo-chromosome 1. The conserved putative repetitive DNA sequence found through informatics analysis showed an extra intercalary band in one arm of pseudo-chromosome 1 in fluorescence the in situ hybridisation result. The results are a basis for establishing a North-South orientation of E. guineensis pseudo-chromosome. Furthermore the ability of the newly developed markers for distinguishing both Elaeis species with in situ hybridisation showed their utility in identifying Elaeis hybrids.
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Abstract
The development of Elaeis guineensis chromosome-arm specific markers is required for linking the genetic sequence and chromosomal maps valuable for comparative studies hybridisation and breeding. Here we aimed to develop strategies exploiting assembled short shotgun-sequence reads to identify markers for in situ hybridisation to identify arms and large-scale organisation of pseudo-chromosome 1. The conserved putative repetitive DNA sequence found through informatics analysis showed an extra intercalary band in one arm of pseudo-chromosome 1 in fluorescence the in situ hybridisation result. The results are a basis for establishing a North-South orientation of E. guineensis pseudo-chromosome. Furthermore the ability of the newly developed markers for distinguishing both Elaeis species with in situ hybridisation showed their utility in identifying Elaeis hybrids.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Elaeis guineensis |
| AGROVOC Term: | Chromosomes |
| AGROVOC Term: | Genetic markers |
| AGROVOC Term: | Hybridization |
| AGROVOC Term: | Identification |
| AGROVOC Term: | Extracts |
| AGROVOC Term: | Purification |
| AGROVOC Term: | Fluorescence |
| AGROVOC Term: | Ribosomal rna |
| AGROVOC Term: | Bioinformatics |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 06:06 |
| URI: | http://webagris.upm.edu.my/id/eprint/24121 |
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