The use of Arabidopsis thaliana model system for testing oil palm promoter: case study on oil palm MT3-A promoter

Zubaidah Ramli, . and Nurniwalis Abdul Wahab, . and Siti Nor Akmar Abdullah, . and Ahmad Parveez Ghulam Kadir, . The use of Arabidopsis thaliana model system for testing oil palm promoter: case study on oil palm MT3-A promoter. pp. 189-196. ISSN 1511-2780

A previous study on transient expression of oil palm tissues has shown that the oil palm metallothionein like type 3 (MT3-A) gene promoter is specifically expressed in the mesocarp and not in other tissues. This study was conducted to determine whether or not Arabidopsis can be used as a model system to study oil palm promoter. Functional characterisation of the oil palm MT3-A promoter was performed using promoter::GUS fusion analysis in transgenic Arabidopsis. The localisation of -glucuronidase (GUS) expression in several different tissues of transgenic Arabidopsis homozygous lines driven by the oil palm MT3-A promoter was determined. Histochemical GUS analysis in transgenic Arabidopsis revealed the highest expression in the cotyledon and hypocotyls as well as at the early stage of plant development and gradually decreased as the plant grew. Lower expression was detected in organs of mature plant and no expression was observed in reproductive tissues. This observation may suggest that MT3-A promoter might be involved during early stage of plant development. Since we use Arabidopsis which is neither a fruit nor mesocarp plant to check mesocarp-specific promoter it might not give a faithful pattern of expression but it may be suited to study oil palm specific promoter derived from other tissues.

Full text available from: Official URL: http://jopr.mpob.gov.my/the-use-of-arabidopsis-tha...

Abstract

A previous study on transient expression of oil palm tissues has shown that the oil palm metallothionein like type 3 (MT3-A) gene promoter is specifically expressed in the mesocarp and not in other tissues. This study was conducted to determine whether or not Arabidopsis can be used as a model system to study oil palm promoter. Functional characterisation of the oil palm MT3-A promoter was performed using promoter::GUS fusion analysis in transgenic Arabidopsis. The localisation of -glucuronidase (GUS) expression in several different tissues of transgenic Arabidopsis homozygous lines driven by the oil palm MT3-A promoter was determined. Histochemical GUS analysis in transgenic Arabidopsis revealed the highest expression in the cotyledon and hypocotyls as well as at the early stage of plant development and gradually decreased as the plant grew. Lower expression was detected in organs of mature plant and no expression was observed in reproductive tissues. This observation may suggest that MT3-A promoter might be involved during early stage of plant development. Since we use Arabidopsis which is neither a fruit nor mesocarp plant to check mesocarp-specific promoter it might not give a faithful pattern of expression but it may be suited to study oil palm specific promoter derived from other tissues.

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Item Type:	Article
AGROVOC Term:	Arabidopsis thaliana
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Gene expression
AGROVOC Term:	Genes
AGROVOC Term:	Genetically engineered organisms
AGROVOC Term:	DNA sequence
AGROVOC Term:	Oil palms
AGROVOC Term:	Oil plants
AGROVOC Term:	Controlled environment
AGROVOC Term:	Agrobacterium
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 07:07
URI:	http://webagris.upm.edu.my/id/eprint/24194

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