Molecular phylogeny of some Malaysian groupers (subfamily: Epinephelinae family: Serranidae) inferred from mitochondrial and nuclear gene sequences

Nor Syakina Nor Rahim, . and Yuzine Esa, . and Aziz Arshad, . Molecular phylogeny of some Malaysian groupers (subfamily: Epinephelinae family: Serranidae) inferred from mitochondrial and nuclear gene sequences. pp. 1-10. ISSN 1823-8556

Groupers are known as one of the important marine and estuarine fishes they are widely distributed in the tropical and subtropical open water. Nevertheless the taxonomy and phylogenetic relationships among wild groupers in Malaysian waters are still poorly studied and need to be further clarified. Thus this study aimed to elucidate the phylogenetic relationship of wild grouper using samples collected from eight coastal areas in Peninsular Malaysia. Samples were identified manually based on their morphological characteristics. Molecular phylogeny was inferred for subfamily Epinephelinae using a combined analysis of the mitochondrial cytochrome b and nuclear Recombination activating gene-1 (RAG-1) genes from 12 species representing three genera of subfamily Epinephelinae. Results from all methods of analyses (Neighbour-joining (NJ) maximum parsimony (MP) maximum likelihood (ML) minimum evolution (ME) and Bayesian analyses (BI)) produced similar tree topologies and strong supported the monophyletic status of the three genera within the subfamily Epinephelinae with genus Plectropomus was basal to the other genera. Phylogenetic analyses also divided genus Epinephelus into two different clades similar to other previous findings. Nevertheless several relationships remain unresolved particularly among morphologically similar species (e.g between E. fario and E. longispinis) and species with limited number of individuals (E. coioides). Overall the findings of this study have managed to provide new insights on the taxonomy phylogeny and genetic diversity of grouper fishes in Peninsular Malaysia which are important for their appropriate sustainable management either for conservation or aquaculture purposes.

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Abstract

Groupers are known as one of the important marine and estuarine fishes they are widely distributed in the tropical and subtropical open water. Nevertheless the taxonomy and phylogenetic relationships among wild groupers in Malaysian waters are still poorly studied and need to be further clarified. Thus this study aimed to elucidate the phylogenetic relationship of wild grouper using samples collected from eight coastal areas in Peninsular Malaysia. Samples were identified manually based on their morphological characteristics. Molecular phylogeny was inferred for subfamily Epinephelinae using a combined analysis of the mitochondrial cytochrome b and nuclear Recombination activating gene-1 (RAG-1) genes from 12 species representing three genera of subfamily Epinephelinae. Results from all methods of analyses (Neighbour-joining (NJ) maximum parsimony (MP) maximum likelihood (ML) minimum evolution (ME) and Bayesian analyses (BI)) produced similar tree topologies and strong supported the monophyletic status of the three genera within the subfamily Epinephelinae with genus Plectropomus was basal to the other genera. Phylogenetic analyses also divided genus Epinephelus into two different clades similar to other previous findings. Nevertheless several relationships remain unresolved particularly among morphologically similar species (e.g between E. fario and E. longispinis) and species with limited number of individuals (E. coioides). Overall the findings of this study have managed to provide new insights on the taxonomy phylogeny and genetic diversity of grouper fishes in Peninsular Malaysia which are important for their appropriate sustainable management either for conservation or aquaculture purposes.

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Item Type:	Article
AGROVOC Term:	Serranidae
AGROVOC Term:	Groupers
AGROVOC Term:	Phylogeny
AGROVOC Term:	Gene sequence
AGROVOC Term:	Fishes
AGROVOC Term:	Coastal area
AGROVOC Term:	Purification
AGROVOC Term:	Electrophoresis
AGROVOC Term:	DNA sequence
AGROVOC Term:	Plectropomus leopardus
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 07:08
URI:	http://webagris.upm.edu.my/id/eprint/24217

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