RNA from fresh frozen cryosections of oil palm inflorescences is superior to FFPE sections

Norazlin Abdul Aziz, . and Parameswari Namasivayam, . and Meilina Ong Abdullah, . and Chai Sook-Keat, . and Ho Chai-Ling, . and Ooi Siaw-Eng, . RNA from fresh frozen cryosections of oil palm inflorescences is superior to FFPE sections. pp. 154-160. ISSN 1511-2780

Formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissue sections are valuable sources for histology and molecular studies. However FFPE tissue sections usually provide poor quality RNA that is unsuitable for most downstream molecular applications. In this study tissue preparation for cryosectioning and RNA extraction protocols were optimised for FF oil palm inflorescences. FF tissue treated with sucrose offered good cellular image resolution and more importantly good RNA quality. Higher RNA quality with good RIN values (6) was obtained from FF sections compared to the fragmented RNA from FFPE tissue sections. However FFPE sections provided better resolution in terms of cellular morphology. The quality of cellular morphology of the FF sections was moderate but was sufficient to distinguish the different cell types. Hence FF oil palm inflorescences is a better choice for downstream transcriptomics studies that require tissue sections without compromising the cytological details.

Full text available from: Official URL: http://jopr.mpob.gov.my/rna-from-fresh-frozen-cryo...

Abstract

Formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissue sections are valuable sources for histology and molecular studies. However FFPE tissue sections usually provide poor quality RNA that is unsuitable for most downstream molecular applications. In this study tissue preparation for cryosectioning and RNA extraction protocols were optimised for FF oil palm inflorescences. FF tissue treated with sucrose offered good cellular image resolution and more importantly good RNA quality. Higher RNA quality with good RIN values (6) was obtained from FF sections compared to the fragmented RNA from FFPE tissue sections. However FFPE sections provided better resolution in terms of cellular morphology. The quality of cellular morphology of the FF sections was moderate but was sufficient to distinguish the different cell types. Hence FF oil palm inflorescences is a better choice for downstream transcriptomics studies that require tissue sections without compromising the cytological details.

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Item Type:	Article
AGROVOC Term:	Oil palms
AGROVOC Term:	Inflorescences
AGROVOC Term:	RNA
AGROVOC Term:	Cell structure
AGROVOC Term:	Freezing
AGROVOC Term:	Isolation
AGROVOC Term:	Extraction
AGROVOC Term:	Gel electrophoresis
AGROVOC Term:	Butanol
AGROVOC Term:	Ethanol
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/24304

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