Evaluation of oil palm leaf-specific promoter (LSP1) activity for expressing PHB genes in Arabidopsis thaliana

Nor Hanin Ayub, . and Abdul Masani Mat Yunus, . and Ahmad Parveez Ghulam Kadir, . Evaluation of oil palm leaf-specific promoter (LSP1) activity for expressing PHB genes in Arabidopsis thaliana. pp. 1-9. ISSN 1511-2780

Production of polyhydroxybutyrate (PHB) a biodegradable plastic in plant has been studied all over the world. We have constructed a transformation vector pLSP15 carrying the PHB biosynthesis genes. Each of PHB genes was driven by an oil palm leaf-specific promoter (LSP1). This transformation vector was later transformed into Arabidopsis thaliana and successfully produced T3 putative transgenic plants. The mature T3 generation was analysed by Reverse Transcription-qPCR (RT-qPCR) to test the capability of a monocotyledon oil palm LSP1 promoter in driving the expression of the PHB genes in a dicotyledon A. thaliana plant. Expression of all PHB genes i.e. phbA (2.32) phbB (1.53) and phbC (1.47) relative to the wild-type were detected in the plant. Nile blue A staining demonstrated that the PHB polymer was successfully produced throughout the developmental stage of the transgenic A. thaliana plant without any deleterious effects. The results demonstrated that the oil palm LSP1 promoter could drive the expression of the PHB biosynthesis genes in plants.

Full text available from: Official URL: http://jopr.mpob.gov.my/evaluation-of-oil-palm-lea...

Abstract

Production of polyhydroxybutyrate (PHB) a biodegradable plastic in plant has been studied all over the world. We have constructed a transformation vector pLSP15 carrying the PHB biosynthesis genes. Each of PHB genes was driven by an oil palm leaf-specific promoter (LSP1). This transformation vector was later transformed into Arabidopsis thaliana and successfully produced T3 putative transgenic plants. The mature T3 generation was analysed by Reverse Transcription-qPCR (RT-qPCR) to test the capability of a monocotyledon oil palm LSP1 promoter in driving the expression of the PHB genes in a dicotyledon A. thaliana plant. Expression of all PHB genes i.e. phbA (2.32) phbB (1.53) and phbC (1.47) relative to the wild-type were detected in the plant. Nile blue A staining demonstrated that the PHB polymer was successfully produced throughout the developmental stage of the transgenic A. thaliana plant without any deleterious effects. The results demonstrated that the oil palm LSP1 promoter could drive the expression of the PHB biosynthesis genes in plants.

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Item Type:	Article
AGROVOC Term:	Arabidopsis thaliana
AGROVOC Term:	Oil palm
AGROVOC Term:	Biosynthesis
AGROVOC Term:	Gene expression
AGROVOC Term:	Genes
AGROVOC Term:	Isolation
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	Ribonucleic acid
AGROVOC Term:	Reverse transcription
AGROVOC Term:	Genetic transformation
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/24319

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