Molecular technique identification of the microbial population in palm oil mill effluent (POME)

Awang Ahmad Sallehin Awang Hussaini, . and Lau Seng, . and Kasing Apun, . and Leslie Bilung, . and Chan Christy Sien Wei, . and Micky Vincent, . and Azham Zulkarnain, . Molecular technique identification of the microbial population in palm oil mill effluent (POME). pp. 293-298. ISSN 1511-2780

Bioconversion of palm oil mill effluent (POME) to generate methane gas via anaerobic digestion involves a consortium of microbes which are responsible in several steps of the biodegradation process. In this study the microbial community from a selected POME was characterised via molecular techniques and through culture-based plating in order to determine their composition and subsequently understand their function in the anaerobic community. Genomic DNA of the microbial community was extracted using direct extraction technique followed by PCR targeting the 16S rDNA region. Distinct fragments of approximately 1100 bp in sizes were successfully amplified using PCR and cloned onto Escherichia coli XL-1 Blue. Upon sequencing of the fragments BLAST queries identified the bacteria as Thermoanaerobacterium sp. In addition two other bacterial species were successfully isolated from the POME by culturing on DVS agar. The sequencing result of these bacterial isolates showed both isolates belonged to the Bacillus genus. By understanding the bacterial community present in the POME this will lead to the improvement of the anaerobic digestion process to enhance the production of biogas such as methane.

Full text available from: Official URL: http://jopr.mpob.gov.my/molecular-technique-identi...

Abstract

Bioconversion of palm oil mill effluent (POME) to generate methane gas via anaerobic digestion involves a consortium of microbes which are responsible in several steps of the biodegradation process. In this study the microbial community from a selected POME was characterised via molecular techniques and through culture-based plating in order to determine their composition and subsequently understand their function in the anaerobic community. Genomic DNA of the microbial community was extracted using direct extraction technique followed by PCR targeting the 16S rDNA region. Distinct fragments of approximately 1100 bp in sizes were successfully amplified using PCR and cloned onto Escherichia coli XL-1 Blue. Upon sequencing of the fragments BLAST queries identified the bacteria as Thermoanaerobacterium sp. In addition two other bacterial species were successfully isolated from the POME by culturing on DVS agar. The sequencing result of these bacterial isolates showed both isolates belonged to the Bacillus genus. By understanding the bacterial community present in the POME this will lead to the improvement of the anaerobic digestion process to enhance the production of biogas such as methane.

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Item Type:	Article
AGROVOC Term:	Palm oils
AGROVOC Term:	Effluents
AGROVOC Term:	Anaerobic digestion
AGROVOC Term:	Anaerobic treatment
AGROVOC Term:	Escherichia coli
AGROVOC Term:	DNA
AGROVOC Term:	Extraction
AGROVOC Term:	Isolation
AGROVOC Term:	Cloning
AGROVOC Term:	Polymerase chain reaction
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 07:23
URI:	http://webagris.upm.edu.my/id/eprint/24334

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