Digital gene expression of water-stressed Phyllanthus urinaria (Dukung anak)

Khairun Hisam Nasir, . and Maheswary Vellupillai, . and Vasanthi Sivarajah, . and Nur Qistina Othman, . Digital gene expression of water-stressed Phyllanthus urinaria (Dukung anak). pp. 121-136. ISSN 1394-9829

In this study we investigated the up- and down-regulation of genes involved in the secondary metabolite biosynthesis pathways in mature dukung anak (Phyllanthus urinaria) plants known to produce phytochemicals. Phytochemicals could contribute to human health by subjecting them to drought stress under net house conditions. Our raw data analyses using digital gene expression (DGE) technology showed that the number of sequence tags in the water-stressed plants was 1.9 folds more than in the control plants. Blast analysis showed that more than 90 of these sequences matched to the annotated sequences in the plantTA database with an up- or down-regulation of several genes in the main isoprenoid shikimate fatty acids steroids and amino acids biosynthesis pathways in the water-stressed plants. Several enzymes were induced after water-stress but at a low frequency while some were either completely suppressed or showed no change in their expression. Gene Ontology (GO) analysis at level 2 revealed a significantly higher number of annotated sequences (E-value 10“6) in the water-stressed plants in each of the biological process molecular function and cellular component categories compared to the control plants. The results clearly indicated the beneficial effect of water-stress in increasing the production of several useful secondary metabolites in P. urinaria plants.

Full text available from: Official URL: http://jtafs.mardi.gov.my/jtafs/44-1/digital.pdf

Abstract

In this study we investigated the up- and down-regulation of genes involved in the secondary metabolite biosynthesis pathways in mature dukung anak (Phyllanthus urinaria) plants known to produce phytochemicals. Phytochemicals could contribute to human health by subjecting them to drought stress under net house conditions. Our raw data analyses using digital gene expression (DGE) technology showed that the number of sequence tags in the water-stressed plants was 1.9 folds more than in the control plants. Blast analysis showed that more than 90 of these sequences matched to the annotated sequences in the plantTA database with an up- or down-regulation of several genes in the main isoprenoid shikimate fatty acids steroids and amino acids biosynthesis pathways in the water-stressed plants. Several enzymes were induced after water-stress but at a low frequency while some were either completely suppressed or showed no change in their expression. Gene Ontology (GO) analysis at level 2 revealed a significantly higher number of annotated sequences (E-value 10“6) in the water-stressed plants in each of the biological process molecular function and cellular component categories compared to the control plants. The results clearly indicated the beneficial effect of water-stress in increasing the production of several useful secondary metabolites in P. urinaria plants.

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Item Type:	Article
AGROVOC Term:	Phyllanthus
AGROVOC Term:	Gene expression
AGROVOC Term:	Water stress
AGROVOC Term:	Secondary metabolites
AGROVOC Term:	Biosynthesis
AGROVOC Term:	Drought stress
AGROVOC Term:	Isoprenoids
AGROVOC Term:	Fatty acids
AGROVOC Term:	Steroids
AGROVOC Term:	Amino acids
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24403

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