Citation
Khairul Asfamawi Khulidin, . and Noraini Samat, . and Darah Ibrahim, . Isolation screening and identification of mannanase producer microorganisms. pp. 169-177. ISSN 1394-9829
Abstract
Palm kernel cake (PKC) is used widely in the animal feed industry but has limited use in poultry feed due to its high fibre and low protein contents. The major component of the fibre is mannan which is insoluble and difficult to digest. Therefore isolation of local potential microbes capable of degrading mannan was explored from various sources such as peat soil rotten oil palm trunks and raw PKC. The isolates were screened based on the clearing zone method on selective agar media containing Azo-carob-galactomannan as substrate. A total of 36 isolates were screened and only 13 had a clear zone ranging from 3.42 0.02 mm to 5.44 0.06 mm which were used for further analysis. These isolates were incubated in shake flasks at 35 C for 48 h with 10 g/litre PKC as substrate. The best enzymes producer was isolate IBRL F16.A4 with specific mannanase enzyme acitivity of 17.82 0.05 U/mg production of glucosamine at 9.84 0.11 mg/g and mannose at 9.54 0.06 mg/g. The isolate IBRL F16.A4 was identified as Aspergillus niger IBRL F16.A4 using a scanning electron microscope.
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Abstract
Palm kernel cake (PKC) is used widely in the animal feed industry but has limited use in poultry feed due to its high fibre and low protein contents. The major component of the fibre is mannan which is insoluble and difficult to digest. Therefore isolation of local potential microbes capable of degrading mannan was explored from various sources such as peat soil rotten oil palm trunks and raw PKC. The isolates were screened based on the clearing zone method on selective agar media containing Azo-carob-galactomannan as substrate. A total of 36 isolates were screened and only 13 had a clear zone ranging from 3.42 0.02 mm to 5.44 0.06 mm which were used for further analysis. These isolates were incubated in shake flasks at 35 C for 48 h with 10 g/litre PKC as substrate. The best enzymes producer was isolate IBRL F16.A4 with specific mannanase enzyme acitivity of 17.82 0.05 U/mg production of glucosamine at 9.84 0.11 mg/g and mannose at 9.54 0.06 mg/g. The isolate IBRL F16.A4 was identified as Aspergillus niger IBRL F16.A4 using a scanning electron microscope.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Isolation |
AGROVOC Term: | Screening tests |
AGROVOC Term: | Identification |
AGROVOC Term: | Palm kernel cake |
AGROVOC Term: | Animal feeding |
AGROVOC Term: | Poultry |
AGROVOC Term: | Fibre content |
AGROVOC Term: | Protein content |
AGROVOC Term: | Mannans |
AGROVOC Term: | Microbes |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 24 Apr 2025 06:29 |
URI: | http://webagris.upm.edu.my/id/eprint/24462 |
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