Identification of phytochemicals and the associated genes in Eksotika papaya at ripening index 5 using functional genomics

Nur Qistina Othman, . and Maheswary Vellupillai, . and Vasanthi Sivarajah, . and Sanimah Simoh, . and Johari Sarip, . Identification of phytochemicals and the associated genes in Eksotika papaya at ripening index 5 using functional genomics. pp. 283-308. ISSN 1394-9829

Phytochemicals and the associated genes involved in primary and secondary metabolite pathways at ripening index 5 of Carica papaya var. Eksotika were investigated using GC-MS based metabolomics approach and mRNA pairedend sequencing. A total of 54 metabolites were identified for polar and nonpolar compounds belonging to amino and organic acids saturated/unsaturated fatty acids sugars/sugar alcohols simple phenolics vitamins and sterols and alkanes. Ascorbic acid (vitamin C) - and -tocopherols (vitamin E) and plant sterols such as stigmasterol campesterol and -sitosterol were among the metabolites identified in this study. A total of 48 765 putative unigenes were obtained from mRNA paired-end sequencing of which 59 (28 736) showed significant (E-value 10“5) expression to enzymes in the non-redundant (nr) database. Of these only 4.9 (1 413) were predicted to be potentially involved in the biosynthesis and metabolism of primary and secondary metabolites based on KEGG pathway analysis. These include carotenoids fruit volatiles including mono- and diterpenoids stillbenoids flavonoids flavones and flavonols anthocyanins phenylpropanoids betalains isothiocyanates steroids saturated and unsaturated fatty acids sugars and sugar alcohols brassinosteroids amino- and organic acids abscisic acid laticifers and proteases. GO analysis identified 67 of the unigenes in the molecular function category to be involved in antioxidant activity. By mapping to the KEGG pathway 29 metabolites were identified to have direct association with several of the predicted papaya unigenes including metabolites belonging to amino and organic acids sugars fatty acids and plant sterols.

Full text available from: Official URL: http://jtafs.mardi.gov.my/jtafs/41-2/Eksotika%20pa...

Abstract

Phytochemicals and the associated genes involved in primary and secondary metabolite pathways at ripening index 5 of Carica papaya var. Eksotika were investigated using GC-MS based metabolomics approach and mRNA pairedend sequencing. A total of 54 metabolites were identified for polar and nonpolar compounds belonging to amino and organic acids saturated/unsaturated fatty acids sugars/sugar alcohols simple phenolics vitamins and sterols and alkanes. Ascorbic acid (vitamin C) - and -tocopherols (vitamin E) and plant sterols such as stigmasterol campesterol and -sitosterol were among the metabolites identified in this study. A total of 48 765 putative unigenes were obtained from mRNA paired-end sequencing of which 59 (28 736) showed significant (E-value 10“5) expression to enzymes in the non-redundant (nr) database. Of these only 4.9 (1 413) were predicted to be potentially involved in the biosynthesis and metabolism of primary and secondary metabolites based on KEGG pathway analysis. These include carotenoids fruit volatiles including mono- and diterpenoids stillbenoids flavonoids flavones and flavonols anthocyanins phenylpropanoids betalains isothiocyanates steroids saturated and unsaturated fatty acids sugars and sugar alcohols brassinosteroids amino- and organic acids abscisic acid laticifers and proteases. GO analysis identified 67 of the unigenes in the molecular function category to be involved in antioxidant activity. By mapping to the KEGG pathway 29 metabolites were identified to have direct association with several of the predicted papaya unigenes including metabolites belonging to amino and organic acids sugars fatty acids and plant sterols.

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Item Type:	Article
AGROVOC Term:	Papayas
AGROVOC Term:	Carica papaya
AGROVOC Term:	genomics
AGROVOC Term:	Genes
AGROVOC Term:	Identification
AGROVOC Term:	Ripening
AGROVOC Term:	Bioinformatics
AGROVOC Term:	Secondary metabolites
AGROVOC Term:	Metabolites
AGROVOC Term:	RNA sequence
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24474

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