Differentially expressed scent-related genes in early bloom Michelia alba

Nurul Aishah Yahya, . and Sanimah Simoh, . and Vasanthi Sivarajah, . and Maheswary Vellupillai, . and Yong Sock Hwa, . Differentially expressed scent-related genes in early bloom Michelia alba. pp. 191-201. ISSN 1394-9829

Two developmental stages of Michelia alba (cempaka putih) flower namely the flower bud stage (S1) (as driver) and early flower development stage (S6) (as tester) were used in this subtractive hybridization study to investigate the scent producing genes at S6. In total 16 scent-related genes were identified six of which belonged to the monoterpene fragrance biosynthesis pathway while the remaining belonged to the sesquiterpene phenylpropanoid/benzenoids and the fatty acid derivatives fragrance biosynthesis pathways. The most abundantly expressed was aromatic-L-amino acid decarboxylase followed by monoterpene glucosyltransferase benzoyl-CoA: benzyl alcohol benzoyl transferase methyl jasmonate esterase cytochrome P450 monoxygenase (-)-germacrene D synthase NADPH dehydrogenase carboxylesterase esterase/lipase/thioesterase 10-hydroxygeraniol oxidoreductase salicyclic acid methyltransferase sesquiterpene cyclase orcinol O-methyltransferase cytochrome P450 and eugenol synthase 2. Several metabolic and scent-related enzymes were also identified including 1-D-deoxyxylulose-5-phosphate synthase (DXPS) geranyl diphosphate synthase (GDPS) lipoxygenase heat shock protein myb-transcription factor and cold-induced glucosyl transferase. The up-regulation of DXPS and GDPS from early to full bloom stage indicated that monoterpene fragrance biosynthesis pathway was the predominant pathway. From the data obtained we further elucidated the monoterpene fragrance biosynthesis pathway and also showed the co-existence of three other scent biosynthesis pathways namely sesquiterpene phenylpropanoid/benzenoids and the fatty acid derivatives during the development of this local flower species.

Full text available from: Official URL: http://jtafs.mardi.gov.my/jtafs/39-2/Michelia%20al...

Abstract

Two developmental stages of Michelia alba (cempaka putih) flower namely the flower bud stage (S1) (as driver) and early flower development stage (S6) (as tester) were used in this subtractive hybridization study to investigate the scent producing genes at S6. In total 16 scent-related genes were identified six of which belonged to the monoterpene fragrance biosynthesis pathway while the remaining belonged to the sesquiterpene phenylpropanoid/benzenoids and the fatty acid derivatives fragrance biosynthesis pathways. The most abundantly expressed was aromatic-L-amino acid decarboxylase followed by monoterpene glucosyltransferase benzoyl-CoA: benzyl alcohol benzoyl transferase methyl jasmonate esterase cytochrome P450 monoxygenase (-)-germacrene D synthase NADPH dehydrogenase carboxylesterase esterase/lipase/thioesterase 10-hydroxygeraniol oxidoreductase salicyclic acid methyltransferase sesquiterpene cyclase orcinol O-methyltransferase cytochrome P450 and eugenol synthase 2. Several metabolic and scent-related enzymes were also identified including 1-D-deoxyxylulose-5-phosphate synthase (DXPS) geranyl diphosphate synthase (GDPS) lipoxygenase heat shock protein myb-transcription factor and cold-induced glucosyl transferase. The up-regulation of DXPS and GDPS from early to full bloom stage indicated that monoterpene fragrance biosynthesis pathway was the predominant pathway. From the data obtained we further elucidated the monoterpene fragrance biosynthesis pathway and also showed the co-existence of three other scent biosynthesis pathways namely sesquiterpene phenylpropanoid/benzenoids and the fatty acid derivatives during the development of this local flower species.

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Item Type:	Article
AGROVOC Term:	Michelia
AGROVOC Term:	Flowers
AGROVOC Term:	Developmental stages
AGROVOC Term:	Hybridization
AGROVOC Term:	Scent
AGROVOC Term:	Genes
AGROVOC Term:	Monoterpenes
AGROVOC Term:	Fragrance
AGROVOC Term:	Biosynthesis
AGROVOC Term:	Sesquiterpenoids
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24525

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