Citation
Matthews Stella, . and Suhaimi Masduki, . Selection of suitable growth medium for free-living diazotrophs isolated from compost. pp. 211-219. ISSN 1394-9829
Abstract
Four free-living nitrogen fixing bacteria namely NC 2 NC 4 NC 10 and NC 11 were tested for nitrogenase activity. NC 10 performed the highest nitrogenase activity (3.01 x 10“3 mol/h/ml) followed by NC 4 (6.84 x 10“4 mol/h/ml) NC 2 (2.36 x 10“5 mol/h/ml) and NC 11 (7.48 x 10“5 mol/h/ml). The nitrogenase activity of mix culture was recorded as 7.48 x 10“5 mol/h/ml. Beijerinckia medium was the most suitable growth medium for all four organisms followed by Derxia medium and Ashbys medium. In these three mediums high microbial density was obtained in a short period. The optimal growth was achieved within 48 h. Beijerinckia medium showed the highest O.D reading 1.326 A at 24 h. This was followed by Derxia medium 1.135 A and Ashbys medium 0.811 A.
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Abstract
Four free-living nitrogen fixing bacteria namely NC 2 NC 4 NC 10 and NC 11 were tested for nitrogenase activity. NC 10 performed the highest nitrogenase activity (3.01 x 10“3 mol/h/ml) followed by NC 4 (6.84 x 10“4 mol/h/ml) NC 2 (2.36 x 10“5 mol/h/ml) and NC 11 (7.48 x 10“5 mol/h/ml). The nitrogenase activity of mix culture was recorded as 7.48 x 10“5 mol/h/ml. Beijerinckia medium was the most suitable growth medium for all four organisms followed by Derxia medium and Ashbys medium. In these three mediums high microbial density was obtained in a short period. The optimal growth was achieved within 48 h. Beijerinckia medium showed the highest O.D reading 1.326 A at 24 h. This was followed by Derxia medium 1.135 A and Ashbys medium 0.811 A.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Composts |
| AGROVOC Term: | Diazotrophs |
| AGROVOC Term: | Nitrogen fixing bacteria |
| AGROVOC Term: | Nitrogenase |
| AGROVOC Term: | Nitrogen fixation |
| AGROVOC Term: | Isolation |
| AGROVOC Term: | Acetylene |
| AGROVOC Term: | Ionization |
| AGROVOC Term: | Optical density |
| AGROVOC Term: | Protein isolates |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/24551 |
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