Induction and morphogenesis of Phalaenopsis callus

Ling Adrain Chieng Kuang, . and Yap Chai Peng, . and Mohd. Shaib Jaafar, . and Vilasini Pillai, . Induction and morphogenesis of Phalaenopsis callus. pp. 147-152. ISSN 1394-9829

Protocorm-like body (PLB) segments of Phalaenopsis var. Hawaiian Clouds x Phalaenopsis Carmelas Dream cultured on half strength Murashige and Skoog medium supplemented with different concentrations of sucrose were maintained in the dark at 25 2 C. Friable callus white to brownish-white in colour were successfully induced on medium containing 30 g/litre of sucrose. Induction of PLBs from callus was carried out on medium supplemented with different combinations of sucrose and maltose at concentration levels between 0 and 10 g/litre. New Dogashima medium (NDM) containing 1 mg/litre of thidiazuron (TDZ) and solidified with 2.8 g/litre of Gelrite was used as basal medium. Callus formed PLBs on all medium supplemented with 5 and 10 g/litre of maltose except those on medium with 10 g/litre of sucrose. An average of 52.5 of callus formed PLBs on medium supplemented with 10 g/litre of maltose without sucrose. Formation of PLBs occurred in all the clumps of callus cultured on medium with addition of 10 g/litre of maltose alone. PLBs derived from the callus were able to regenerate into plantlets.

Full text available from: Official URL: http://ejtafs.mardi.gov.my/jtafs/35-1/Phalaenopsis...

Abstract

Protocorm-like body (PLB) segments of Phalaenopsis var. Hawaiian Clouds x Phalaenopsis Carmelas Dream cultured on half strength Murashige and Skoog medium supplemented with different concentrations of sucrose were maintained in the dark at 25 2 C. Friable callus white to brownish-white in colour were successfully induced on medium containing 30 g/litre of sucrose. Induction of PLBs from callus was carried out on medium supplemented with different combinations of sucrose and maltose at concentration levels between 0 and 10 g/litre. New Dogashima medium (NDM) containing 1 mg/litre of thidiazuron (TDZ) and solidified with 2.8 g/litre of Gelrite was used as basal medium. Callus formed PLBs on all medium supplemented with 5 and 10 g/litre of maltose except those on medium with 10 g/litre of sucrose. An average of 52.5 of callus formed PLBs on medium supplemented with 10 g/litre of maltose without sucrose. Formation of PLBs occurred in all the clumps of callus cultured on medium with addition of 10 g/litre of maltose alone. PLBs derived from the callus were able to regenerate into plantlets.

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Item Type:	Article
AGROVOC Term:	Phalaenopsis
AGROVOC Term:	Morphogenesis
AGROVOC Term:	Tissue culture
AGROVOC Term:	Sucrose
AGROVOC Term:	Callus
AGROVOC Term:	Maltose
AGROVOC Term:	Plantlets (seedlings)
AGROVOC Term:	Thidiazuron
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24585

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