Isolation of a partial cDNA clone coding for Ganoderma boninense pde

Idris Abu Seman, . and Lim F. H., . and Ghulam Kadir Ahmad Parveez, . and Omar A. Rasid, . and Nor Fakhrana Iskandar, . Isolation of a partial cDNA clone coding for Ganoderma boninense pde. pp. 265-269. ISSN 1511-2780

Ganoderma is white rot fungus that causes a major disease in oil palm. Control measures that have been introduced have not been able to curb the disease effectively. Efforts are underway to uncover more information on the Ganoderma-oil palm interaction especially at the molecular level. During plant infection a few genes are highly expressed in fungus and have been suggested to be related or involved in fungal pathogenicity. One of these genes is the phospholipid-transporting ATPase (pde). In Magnaporthe grisea its protein has been suggested to be associated with hyphae development and proliferation. Degenerate primers were designed based on the conserved regions of the gene. These primers were successfully used to amplify a 544 bp cDNA fragment from Ganoderma boninense. DNA sequence analysis showed the fragment has about 80 similarity to other plant pathogenic fungus pde genes.

Full text available from: Official URL: http://jopr.mpob.gov.my/wp-content/uploads/2014/10...

Abstract

Ganoderma is white rot fungus that causes a major disease in oil palm. Control measures that have been introduced have not been able to curb the disease effectively. Efforts are underway to uncover more information on the Ganoderma-oil palm interaction especially at the molecular level. During plant infection a few genes are highly expressed in fungus and have been suggested to be related or involved in fungal pathogenicity. One of these genes is the phospholipid-transporting ATPase (pde). In Magnaporthe grisea its protein has been suggested to be associated with hyphae development and proliferation. Degenerate primers were designed based on the conserved regions of the gene. These primers were successfully used to amplify a 544 bp cDNA fragment from Ganoderma boninense. DNA sequence analysis showed the fragment has about 80 similarity to other plant pathogenic fungus pde genes.

[error in script]

Item Type:	Article
AGROVOC Term:	Ganoderma
AGROVOC Term:	Clones
AGROVOC Term:	Isolation
AGROVOC Term:	Fungi
AGROVOC Term:	Plant diseases
AGROVOC Term:	Disease control
AGROVOC Term:	Disease control methods
AGROVOC Term:	Oil palms
AGROVOC Term:	Infection
AGROVOC Term:	Fungal diseases
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24594

Actions (login required)

View Item