Citation
Sharif R., . and Chan K. M., . and Oii T. C., . and Mohammad N. F., . Cytotoxicity and genotoxicity evaluation of stevioside on CCD18Co and HCT 116 cell lines. pp. 341-345. ISSN ISSN(Online):22317546
Abstract
Recent findings showed that stevioside can demonstrate anti-cancer property in selected cell lines. In this study the cytotoxicity and genotoxicity of stevioside were examined on human colon carcinoma cell HCT 116 (targeted cell) and human colon derived CD18Co myofibroblast cell lines (non-targeted cell) using the MTT (3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltettrazolium bromide) assay and alkaline comet assay respectively. Result demonstrated that stevioside induced cell death on both HCT 116 and CCD18Co cell lines only at the highest concentration 200 M by causing not more than 20 and 30 percent of cell death on CCD18Co and HCT 116 cell lines respectively (p0.05). The DNA strand break measured via alkaline comet assay showed that it did not cause DNA damage at the same concentration on CCD18Co as well as in HCT 116 cell lines (p0.05). In conclusion stevioside did not exhibit cytotoxic and genotoxic effect on HCT 116 and CCD18Co cell lines respectively hence secured its uses as a non-caloric sweetener.
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Abstract
Recent findings showed that stevioside can demonstrate anti-cancer property in selected cell lines. In this study the cytotoxicity and genotoxicity of stevioside were examined on human colon carcinoma cell HCT 116 (targeted cell) and human colon derived CD18Co myofibroblast cell lines (non-targeted cell) using the MTT (3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltettrazolium bromide) assay and alkaline comet assay respectively. Result demonstrated that stevioside induced cell death on both HCT 116 and CCD18Co cell lines only at the highest concentration 200 M by causing not more than 20 and 30 percent of cell death on CCD18Co and HCT 116 cell lines respectively (p0.05). The DNA strand break measured via alkaline comet assay showed that it did not cause DNA damage at the same concentration on CCD18Co as well as in HCT 116 cell lines (p0.05). In conclusion stevioside did not exhibit cytotoxic and genotoxic effect on HCT 116 and CCD18Co cell lines respectively hence secured its uses as a non-caloric sweetener.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Stevioside |
| AGROVOC Term: | Stevia |
| AGROVOC Term: | Medicinal plants |
| AGROVOC Term: | Sugar substitutes |
| AGROVOC Term: | Cytotoxicity |
| AGROVOC Term: | Genotoxicity |
| AGROVOC Term: | Evaluation |
| AGROVOC Term: | Pharmacology |
| AGROVOC Term: | Cancer (disease) |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/24598 |
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