The use of palm kernel cake in the production of conidia and blastospores of Metarhizium anisopliae var. major for control of Oryctes rhinoceros

Ramle Moslim, . and Norman Kamarudin, . The use of palm kernel cake in the production of conidia and blastospores of Metarhizium anisopliae var. major for control of Oryctes rhinoceros. pp. 133-139. ISSN 1511-2780

The yield of conidia and blastospores of Metarhizium anisopliae var. major produced on maize supplemented with palm kernel cake (PKC) was estimated. In maize supplemented with 5 g and 10 g of PKC the fungus produced 4.3 g and 5.6 g of conidia respectively. This is significantly higher than the yield of conidia produced on maize alone which was 2.03 g. The fungus produced submerged spores termed as blastospores in a simple liquid medium consisting of glucose and PKC. The formation of blastospores during the fermentation process was monitored. The results showed that the young blastospores are round to ovoid with 5.0-5.5 m in diameter and matured blastospores are commonly ellipsoid with dimensions ranging from 5.0-5.5 m x 12.5-15.5 m. The highest yield of the blastospores was 3.26 x 106 blastospores ml-1 produced at seven days after fermentation. The conidia and blastospores were equally effective in controlling the third instar larvae of the Oryctes rhinoceros. Sixteen days after treatment both inocula killepdad 100 larvae with infection of between 94.3 and 97.1. The LC50 values for both inocula were almost similar at 9.1 days for conidia and 9.5 days for blastospores. This study showed that PKC can be used as a supplement to produce conidia and blastospores of the fungus M. anisopliae var. major and that the blastospores are potential for the biocontrol of the rhinoceros beetle.

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Abstract

The yield of conidia and blastospores of Metarhizium anisopliae var. major produced on maize supplemented with palm kernel cake (PKC) was estimated. In maize supplemented with 5 g and 10 g of PKC the fungus produced 4.3 g and 5.6 g of conidia respectively. This is significantly higher than the yield of conidia produced on maize alone which was 2.03 g. The fungus produced submerged spores termed as blastospores in a simple liquid medium consisting of glucose and PKC. The formation of blastospores during the fermentation process was monitored. The results showed that the young blastospores are round to ovoid with 5.0-5.5 m in diameter and matured blastospores are commonly ellipsoid with dimensions ranging from 5.0-5.5 m x 12.5-15.5 m. The highest yield of the blastospores was 3.26 x 106 blastospores ml-1 produced at seven days after fermentation. The conidia and blastospores were equally effective in controlling the third instar larvae of the Oryctes rhinoceros. Sixteen days after treatment both inocula killepdad 100 larvae with infection of between 94.3 and 97.1. The LC50 values for both inocula were almost similar at 9.1 days for conidia and 9.5 days for blastospores. This study showed that PKC can be used as a supplement to produce conidia and blastospores of the fungus M. anisopliae var. major and that the blastospores are potential for the biocontrol of the rhinoceros beetle.

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Item Type:	Article
AGROVOC Term:	Oryctes rhinoceros
AGROVOC Term:	Conidia
AGROVOC Term:	Palm kernel cake
AGROVOC Term:	Metarhizium anisopliae
AGROVOC Term:	Maize
AGROVOC Term:	Fungus control (diseases)
AGROVOC Term:	Glucose
AGROVOC Term:	Fermentation
AGROVOC Term:	Larvae
AGROVOC Term:	Inoculation
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24622

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