Extraction methods for analysis of oil palm leaf and root proteins by two-dimensional gel electrophoresis

Hasliza Hassan, . and Lau B. Y. C., . and Umi Salamah Ramli, . Extraction methods for analysis of oil palm leaf and root proteins by two-dimensional gel electrophoresis. pp. 54-61. ISSN 1511-2780

Oil palm (Elaeis guineensis Jacq.) is an important crop in Malaysia and as such it has become the second largest export earner for the country. This study ventured into developing suitable gel-based proteomics methods for specific oil palm tissues with the aim of understanding its biological systems. An optimal procedure for extraction of proteins from oil palm root and leaf tissues was developed through evaluations and modifications of three existing methods. The TCA/acetone phenol/ammonium acetate and chloroform/acetone protein extraction approaches were tested on the root and leaf tissues of oil palm. The total protein yields and resulting 2-DGE protein spot profiles revealed that TCA/acetone method is the most effective approach for both tissues. Thus TCA/acetone method will be used to produce total proteins for downstream analyses by mass spectrometry.

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Abstract

Oil palm (Elaeis guineensis Jacq.) is an important crop in Malaysia and as such it has become the second largest export earner for the country. This study ventured into developing suitable gel-based proteomics methods for specific oil palm tissues with the aim of understanding its biological systems. An optimal procedure for extraction of proteins from oil palm root and leaf tissues was developed through evaluations and modifications of three existing methods. The TCA/acetone phenol/ammonium acetate and chloroform/acetone protein extraction approaches were tested on the root and leaf tissues of oil palm. The total protein yields and resulting 2-DGE protein spot profiles revealed that TCA/acetone method is the most effective approach for both tissues. Thus TCA/acetone method will be used to produce total proteins for downstream analyses by mass spectrometry.

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Item Type:	Article
AGROVOC Term:	Oil palm
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Leaf analysis
AGROVOC Term:	Roots
AGROVOC Term:	Extraction
AGROVOC Term:	Gel electrophoresis
AGROVOC Term:	Fruit crops
AGROVOC Term:	Plant tissues
AGROVOC Term:	Leaf tissue analysis
AGROVOC Term:	Tca
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24629

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