First report: isolation of endophytic Trichoderma from oil palm (Elaeis guineensis Jacq.) and their in vitro antagonistic assessment on Ganoderma boninense

Shamala Sundram, . First report: isolation of endophytic Trichoderma from oil palm (Elaeis guineensis Jacq.) and their in vitro antagonistic assessment on Ganoderma boninense. pp. 368-372. ISSN 1511-2780

The isolation of endophytic Trichoderma from different tissues viz. leaf rachis stem and root of oil palm (Elaeis guineensis Jacq.) was investigated in this study. A total of six palms were sampled from two plantation plots and it was found that Trichoderma isolates were specifically colonising the stems and roots of oil palm and none were found in the leaves and rachis. Trichoderma selective media E (TME) was used for isolation. Identification of the isolates was further established using slide cultures. A total of 40 isolates were obtained with 10 from stem and 30 from root samples of oil palm. All isolates were subjected to dual culture assay; a preliminary assay to screen mycoparatisms potential through antagonistic activity against Ganoderma boninense. Subsequently seven potential Trichoderma isolates were tested for their antibiosis properties through poison food agar assay. This is the first report of endophytic Trichoderma isolated from oil palm with biocontrol potential against G. boninense.

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Abstract

The isolation of endophytic Trichoderma from different tissues viz. leaf rachis stem and root of oil palm (Elaeis guineensis Jacq.) was investigated in this study. A total of six palms were sampled from two plantation plots and it was found that Trichoderma isolates were specifically colonising the stems and roots of oil palm and none were found in the leaves and rachis. Trichoderma selective media E (TME) was used for isolation. Identification of the isolates was further established using slide cultures. A total of 40 isolates were obtained with 10 from stem and 30 from root samples of oil palm. All isolates were subjected to dual culture assay; a preliminary assay to screen mycoparatisms potential through antagonistic activity against Ganoderma boninense. Subsequently seven potential Trichoderma isolates were tested for their antibiosis properties through poison food agar assay. This is the first report of endophytic Trichoderma isolated from oil palm with biocontrol potential against G. boninense.

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Item Type:	Article
AGROVOC Term:	Trichoderma
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Oil palms
AGROVOC Term:	Ganoderma
AGROVOC Term:	Isolation
AGROVOC Term:	In vitro experimentation
AGROVOC Term:	Leaf tissue analysis
AGROVOC Term:	Plantations
AGROVOC Term:	Antibiosis
AGROVOC Term:	Antagonism
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24633

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