Citation
Rosimah Nulit, . and Samsul Kamal Rosli, . and Maria Madon, . and Mohd Razik Midin, . and Tarmizi A. H., . Analysis of oil palm clones their suspension calli and regenerants via flow cytometry (FCM) and rDNA-fluorescence in situ hybridisation (rDNA-FISH). pp. 357-367. ISSN 1511-2780
Abstract
Clonal propagation of palms with good traits is desirable for the oil palm industry. In this study flow cytometry (FCM) and 18S-25S ribosomal DNA-fluorescence in situ hybridisation (rDNA-FISH) were utilised to analyse genetic variation in adult clonal palms their respective suspension cultures and regenerant plantlets. The genome sizes estimated by FCM for the four adult clonal palms (using leaf samples from Frond-1) varied from 2C2.590.19 pg to 2.910.14 pg and for their respective regenerants the genome size varied from 2C2.140.21 pg to 3.050.11 pg. The genome size of oil palm suspension cultures could not be analysed by FCM due to the low nuclei population which was less than 1000. The rDNA-FISH analysis showed two hybridisation signals on interphase nuclei of suspension culture calli and regenerant plantlets hence indicating the diploid ploidy level. Adult clonal palms with their suspension culture calli and regenerants therefore showed a similar ploidy level. However the measurement of genome size was found to vary between the adult clonal palms and their regenerants
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Abstract
Clonal propagation of palms with good traits is desirable for the oil palm industry. In this study flow cytometry (FCM) and 18S-25S ribosomal DNA-fluorescence in situ hybridisation (rDNA-FISH) were utilised to analyse genetic variation in adult clonal palms their respective suspension cultures and regenerant plantlets. The genome sizes estimated by FCM for the four adult clonal palms (using leaf samples from Frond-1) varied from 2C2.590.19 pg to 2.910.14 pg and for their respective regenerants the genome size varied from 2C2.140.21 pg to 3.050.11 pg. The genome size of oil palm suspension cultures could not be analysed by FCM due to the low nuclei population which was less than 1000. The rDNA-FISH analysis showed two hybridisation signals on interphase nuclei of suspension culture calli and regenerant plantlets hence indicating the diploid ploidy level. Adult clonal palms with their suspension culture calli and regenerants therefore showed a similar ploidy level. However the measurement of genome size was found to vary between the adult clonal palms and their regenerants
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Oil palms |
| AGROVOC Term: | Clones |
| AGROVOC Term: | Flow cytometry (cells) |
| AGROVOC Term: | Fluorescence |
| AGROVOC Term: | Hybridization |
| AGROVOC Term: | Plant propagation |
| AGROVOC Term: | Plantlets (seedlings) |
| AGROVOC Term: | Palms |
| AGROVOC Term: | Clonal variation |
| AGROVOC Term: | Genomes |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/24634 |
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