Citation
Srinivasa G. T. K., . and Sameena M. T., . and Nishanth K. S., . and Veena K. S., . and Padmakumari A. K. P., . and Reshma M. V., . Development and validation of HPLC method for determination of sugars in palm sap palm syrup sugarcane jaggery and palm jaggery. pp. 649-654. ISSN 2231-7546
Abstract
A simple and reproducible method for the qualitative and quantitative analysis of free mono- (fructose glucose) and disaccharides (sucrose) using a high performance liquid chromatography (HPLC) with refractive index (RI) detector and a NH2 column was developed for matrices rich in monosaccharides (honey) and disaccharides (palm sap palm syrup sugarcane jaggery palm jaggery). The developed HPLC method was validated in terms of their linearity limit of detection and quantification precision and accuracy. Method validation studies showed that 85:15 (v/v) acetonitrile/water (CH3 CN:H2 O) was suitable for matrix containing monosaccharides and 65:35 (v/v) (CH3 CN: H2 O) was suitable for matrix containing disaccharides. The method showed good linearity with determination coefficients exceeding 0.99. Recovery studies indicated that 65 (CH3 CN:H2 O) system was suitable for matrix rich in sucrose (e.g. jaggery syrup etc.).
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Abstract
A simple and reproducible method for the qualitative and quantitative analysis of free mono- (fructose glucose) and disaccharides (sucrose) using a high performance liquid chromatography (HPLC) with refractive index (RI) detector and a NH2 column was developed for matrices rich in monosaccharides (honey) and disaccharides (palm sap palm syrup sugarcane jaggery palm jaggery). The developed HPLC method was validated in terms of their linearity limit of detection and quantification precision and accuracy. Method validation studies showed that 85:15 (v/v) acetonitrile/water (CH3 CN:H2 O) was suitable for matrix containing monosaccharides and 65:35 (v/v) (CH3 CN: H2 O) was suitable for matrix containing disaccharides. The method showed good linearity with determination coefficients exceeding 0.99. Recovery studies indicated that 65 (CH3 CN:H2 O) system was suitable for matrix rich in sucrose (e.g. jaggery syrup etc.).
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Sweeteners |
| AGROVOC Term: | Honey |
| AGROVOC Term: | Glucose syrups |
| AGROVOC Term: | Coconut palm |
| AGROVOC Term: | Sap |
| AGROVOC Term: | Sugarcane juice |
| AGROVOC Term: | Palm sugar |
| AGROVOC Term: | Disaccharides |
| AGROVOC Term: | Glucose syrups |
| AGROVOC Term: | HPLC |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/24692 |
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