Phenotypic and molecular characterisations of lactic acid bacteria isolated from Malaysian fruits

Nur Syahirah Azmi, . and Amalia Mohd Hashim, . Phenotypic and molecular characterisations of lactic acid bacteria isolated from Malaysian fruits. pp. 1599-1612. ISSN 1511-3701

Lactic Acid Bacteria (LAB) are gram-positive catalase-negative and non-spore forming bacteria known to have many advantages such as starter culture in food fermentation as antimicrobial agent and plant growth promoter. Limited information on various LAB present in Malaysian fruits hampers further study to explore their potential as autochthonous inoculants in food fermentation plant disease control and growth promotion. Therefore the objectives of this study are firstly to isolate and identify LAB from honeydew ciku mango and mata kucing by investigating their morphological and biochemical characteristics secondly to determine the identity of the isolates using 16S rRNA gene sequencing and finally to examine phylogenetic relationship of the LAB present in the fruits. The isolates were subjected to Gram staining acidity and catalase tests followed by molecular identification and phylogenetic analysis of the bacteria. Out of 33 isolates eight isolates were gram- positive catalase-negative and acid producers suggesting that they are potentially LAB. 16S rRNA sequencing and NCBI Blast analysis identified the presence of Lactococcus sp. Leuconostoc sp. Weissella sp. and Aerococcus sp. in the fruit samples with sequence identity 94-97. Phylogenetic tree was constructed based on the 16S rRNA sequences using Neighbor-Joining method. This study has assisted in collecting more information about the diversity of LAB in Malaysian fruits which can be further explored in future for their application as bioinoculant in food fermentation or as biocontrol agent and plant growth promoter in agricultural field.

Full text available from: Official URL: http://www.pertanika.upm.edu.my/Pertanika%20PAPERS...

Abstract

Lactic Acid Bacteria (LAB) are gram-positive catalase-negative and non-spore forming bacteria known to have many advantages such as starter culture in food fermentation as antimicrobial agent and plant growth promoter. Limited information on various LAB present in Malaysian fruits hampers further study to explore their potential as autochthonous inoculants in food fermentation plant disease control and growth promotion. Therefore the objectives of this study are firstly to isolate and identify LAB from honeydew ciku mango and mata kucing by investigating their morphological and biochemical characteristics secondly to determine the identity of the isolates using 16S rRNA gene sequencing and finally to examine phylogenetic relationship of the LAB present in the fruits. The isolates were subjected to Gram staining acidity and catalase tests followed by molecular identification and phylogenetic analysis of the bacteria. Out of 33 isolates eight isolates were gram- positive catalase-negative and acid producers suggesting that they are potentially LAB. 16S rRNA sequencing and NCBI Blast analysis identified the presence of Lactococcus sp. Leuconostoc sp. Weissella sp. and Aerococcus sp. in the fruit samples with sequence identity 94-97. Phylogenetic tree was constructed based on the 16S rRNA sequences using Neighbor-Joining method. This study has assisted in collecting more information about the diversity of LAB in Malaysian fruits which can be further explored in future for their application as bioinoculant in food fermentation or as biocontrol agent and plant growth promoter in agricultural field.

[error in script]

Item Type:	Article
AGROVOC Term:	Lactic acid bacteria
AGROVOC Term:	Isolation techniques
AGROVOC Term:	Isolation of microorganisms
AGROVOC Term:	Fruits
AGROVOC Term:	Honeydew
AGROVOC Term:	Mangoes
AGROVOC Term:	Biochemical indicators (organisms)
AGROVOC Term:	Lactococcus lactis
AGROVOC Term:	Leuconostoc
AGROVOC Term:	Aerococcus
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24749

Actions (login required)

View Item