Citation
McKinnon G. E., . and Larcombe M. J., . and Griffin A. R., . and Vaillancourt R. E., . Development of microsatellites using next-generation sequencing for Acacia crassicarpa. pp. 252-258. ISSN 0128-1283
Abstract
Acacia crassicarpa is a tropical forestry species that is increasingly being planted in South-East Asia. Using next- generation sequencing we developed 12 nuclear microsatellite markers for A. crassicarpa and optimised them for assay in three multiplex sets. We tested the 12 loci on 34 A. crassicarpa samples and found that polymorphism ranged from 4 to 8 alleles per locus (average 5.8). The loci were easy to score in terms of binning and their reproducibility and polymerase chain reaction success rate were high. Given their usability and polymorphism we believe that these 12 loci will be useful for DNA profiling and mating system analysis with direct application in breeding programmes and the conservation of wild populations. Six of the markers also amplified products in related A. mangium and A. auriculiformis with four being polymorphic in three samples of each species. Therefore these markers added more broadly to the genomic resources available in Acacia.
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Abstract
Acacia crassicarpa is a tropical forestry species that is increasingly being planted in South-East Asia. Using next- generation sequencing we developed 12 nuclear microsatellite markers for A. crassicarpa and optimised them for assay in three multiplex sets. We tested the 12 loci on 34 A. crassicarpa samples and found that polymorphism ranged from 4 to 8 alleles per locus (average 5.8). The loci were easy to score in terms of binning and their reproducibility and polymerase chain reaction success rate were high. Given their usability and polymorphism we believe that these 12 loci will be useful for DNA profiling and mating system analysis with direct application in breeding programmes and the conservation of wild populations. Six of the markers also amplified products in related A. mangium and A. auriculiformis with four being polymorphic in three samples of each species. Therefore these markers added more broadly to the genomic resources available in Acacia.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Acacia crassicarpa |
| AGROVOC Term: | Forest plantations |
| AGROVOC Term: | Microsatellites |
| AGROVOC Term: | DNA sequence |
| AGROVOC Term: | Silviculture |
| AGROVOC Term: | Genetic resources |
| AGROVOC Term: | Genetic markers |
| AGROVOC Term: | Vegetative reproduction (physiol) |
| AGROVOC Term: | genomics |
| AGROVOC Term: | Genetic improvement |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:29 |
| URI: | http://webagris.upm.edu.my/id/eprint/24781 |
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