First report of Rhizoctonia solani Kuhn. isolated from parthenium weed (Parthenium hysterophorus l.) in Malaysia

Fatimah Kayat, . and Laila Naher, . and Nabilah Sarip, . and S. M. R. Karim, . and Norhafizah M. Z., . First report of Rhizoctonia solani Kuhn. isolated from parthenium weed (Parthenium hysterophorus l.) in Malaysia. pp. 1355-1365. ISSN 1511-3701

Fungal pathogens are useful in biocontrol of parthenium weed (Parthenium hysterophorus L.) for efficient weed management. Although parthenium is a major problem in Malaysia no initiative for developing biocontrol method has been taken yet. Therefore a field study was conducted at Kg. Durian Tunggal Melaka (Latitude: 202055N Longitude: 10201733 E) Malaysia in August 2017 to isolate disease-causing pathogens of parthenium weed. Diseased parthenium leaves with symptoms of yellowish-brown leaf blight and the parthenium stem with reddish-brown cankers on the basal part were collected and examined after they were cultured in potato dextrose agar (PDA) medium. Isolated fungus was identified based on morphological and microscopy analyses. Two isolates UMKRSPL1 and UMKRSPS1 were isolated qualitatively from the infected leaves and stem respectively that yielded dark-brown colonies of sclerotia on PDA. Under the microscopic study the mycelia with multi-nucleolus hyphal cells were noted which were septate and hyaline. The hyphae branched at right and acute angles to the primary hypha. There was no conidium. Pathogenicity of the fungus proved to cause similar symptoms on new fresh parthenium leaf. Based on cultural and morphological characteristics the pathogen was identified as Rhizoctonia solani Kuhn. There is no published study on R. solani isolated from P. hysterophorus in Malaysia. This is the first research on the species found on parthenium weed in the country. The identification of pathogens from parthenium weed would help develop bioherbicide by extracting the toxins produced by the fungus in the media broth for effective control of P. hysterophorus in Malaysia.

Full text available from: Official URL: http://www.pertanika.upm.edu.my/Pertanika%20PAPERS...

Abstract

Fungal pathogens are useful in biocontrol of parthenium weed (Parthenium hysterophorus L.) for efficient weed management. Although parthenium is a major problem in Malaysia no initiative for developing biocontrol method has been taken yet. Therefore a field study was conducted at Kg. Durian Tunggal Melaka (Latitude: 202055N Longitude: 10201733 E) Malaysia in August 2017 to isolate disease-causing pathogens of parthenium weed. Diseased parthenium leaves with symptoms of yellowish-brown leaf blight and the parthenium stem with reddish-brown cankers on the basal part were collected and examined after they were cultured in potato dextrose agar (PDA) medium. Isolated fungus was identified based on morphological and microscopy analyses. Two isolates UMKRSPL1 and UMKRSPS1 were isolated qualitatively from the infected leaves and stem respectively that yielded dark-brown colonies of sclerotia on PDA. Under the microscopic study the mycelia with multi-nucleolus hyphal cells were noted which were septate and hyaline. The hyphae branched at right and acute angles to the primary hypha. There was no conidium. Pathogenicity of the fungus proved to cause similar symptoms on new fresh parthenium leaf. Based on cultural and morphological characteristics the pathogen was identified as Rhizoctonia solani Kuhn. There is no published study on R. solani isolated from P. hysterophorus in Malaysia. This is the first research on the species found on parthenium weed in the country. The identification of pathogens from parthenium weed would help develop bioherbicide by extracting the toxins produced by the fungus in the media broth for effective control of P. hysterophorus in Malaysia.

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Item Type:	Article
AGROVOC Term:	Parthenium
AGROVOC Term:	Weeds
AGROVOC Term:	Rhizoctonia solani
AGROVOC Term:	Pathogens
AGROVOC Term:	Fungi
AGROVOC Term:	Leaf
AGROVOC Term:	Stems
AGROVOC Term:	Isolation of microorganisms
AGROVOC Term:	Biocontrol
AGROVOC Term:	Weed control methods
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24820

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