Gram-positive bacteria with commercial potential from the gastrointestines of Holothuria (Mertensiothuria) leucospilota (timun laut) and Stichopus horrens (gamat) from Malaysian waters

Kamarul Rahim Kamarudin, . and Maryam Mohamed Rehan, . Gram-positive bacteria with commercial potential from the gastrointestines of Holothuria (Mertensiothuria) leucospilota (timun laut) and Stichopus horrens (gamat) from Malaysian waters. pp. 605-620. ISSN 1511-3701

A few studies on the presence of microbes and their association in sea cucumbers can be found to date especially in Apostichopus japonicus. However such studies on Malaysian sea cucumbers and in the gastrointestines of the echinoderms are still lacking. Therefore the aims of this study are to isolate and identify associated bacteria in the gastrointestines of two common species of Malaysian sea cucumbers i.e. Holothuria (Mertensiothuria) leucospilota (Brandt 1835) the most dominant sea cucumber species in Malaysia and Stichopus horrens Selenka 1867 the commercial gamat species. A number of six genera of Gram-positive bacteria representing the order Bacillales and the order Micrococcales i.e. Bacillus Brevibacillus Lysinibacillus Staphylococcus Dermacoccus and Micrococcus are isolated from the gastrointestines of H. leucospilota as suggested by the phylogenetic trees of partial 16S rRNA gene sequences. Meanwhile three genera of Gram-positive bacteria that represent the order Bacillales i.e. Bacillus Brevibacillus and Lysinibacillus are isolated from the gastrointestines of S. horrens. Interestingly 60 of the bacterial species have been known to contain commercial potentials mainly as antibiotic producers and only bacteria with commercial potentials were present in the gastrointestines of S. horrens. In contrast to that bacteria that could be pathogenic were also present in the gastrointestines of H. leucospilota. The presence of all bacteria that have been known to contain commercial potentials in the gastrointestines of S. horrens and more diverse microbial population in H. leucospilota could be due to the higher level of antimicrobial properties in the gastrointestines of S. horrens. However experiments on antibacterial properties of the isolates have to be done and those proven to contain commercial potential can be exploited towards the development of industrial applications in Malaysia.

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Abstract

A few studies on the presence of microbes and their association in sea cucumbers can be found to date especially in Apostichopus japonicus. However such studies on Malaysian sea cucumbers and in the gastrointestines of the echinoderms are still lacking. Therefore the aims of this study are to isolate and identify associated bacteria in the gastrointestines of two common species of Malaysian sea cucumbers i.e. Holothuria (Mertensiothuria) leucospilota (Brandt 1835) the most dominant sea cucumber species in Malaysia and Stichopus horrens Selenka 1867 the commercial gamat species. A number of six genera of Gram-positive bacteria representing the order Bacillales and the order Micrococcales i.e. Bacillus Brevibacillus Lysinibacillus Staphylococcus Dermacoccus and Micrococcus are isolated from the gastrointestines of H. leucospilota as suggested by the phylogenetic trees of partial 16S rRNA gene sequences. Meanwhile three genera of Gram-positive bacteria that represent the order Bacillales i.e. Bacillus Brevibacillus and Lysinibacillus are isolated from the gastrointestines of S. horrens. Interestingly 60 of the bacterial species have been known to contain commercial potentials mainly as antibiotic producers and only bacteria with commercial potentials were present in the gastrointestines of S. horrens. In contrast to that bacteria that could be pathogenic were also present in the gastrointestines of H. leucospilota. The presence of all bacteria that have been known to contain commercial potentials in the gastrointestines of S. horrens and more diverse microbial population in H. leucospilota could be due to the higher level of antimicrobial properties in the gastrointestines of S. horrens. However experiments on antibacterial properties of the isolates have to be done and those proven to contain commercial potential can be exploited towards the development of industrial applications in Malaysia.

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Item Type:	Article
AGROVOC Term:	Sea cucumbers
AGROVOC Term:	Holothuria leucospilota
AGROVOC Term:	Stichopus horrens
AGROVOC Term:	Isolation of microorganisms
AGROVOC Term:	Culture media
AGROVOC Term:	Extraction
AGROVOC Term:	Staphylococcus
AGROVOC Term:	Gastrointestinal agents
AGROVOC Term:	Bacteria
AGROVOC Term:	Bacillus
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24838

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