Genetic diversity and relationship of Sabah traditional rice varieties as revealed by RAPD markers


Citation

Wong Jovita Jun, . and Wah Goh Lucky Poh, . and Noumie @ Loumie Surugau, . and Mariam Abd. Latip and, . and Jiann Chong Eric Tzyy, . and Lee Ping-Chin, . and Zaleha Abdul Aziz, . Genetic diversity and relationship of Sabah traditional rice varieties as revealed by RAPD markers. pp. 177-190. ISSN 1511-3701

Abstract

Sabah also known as North Borneo is one of the states in Malaysia. It is home to many local varieties of rice but the self-sufficiency quotient for rice production is only about 30. Knowledge of the genetic diversity of crops has been utilised to increase crop yields including rice in different countries but the information regarding the genetic diversity of Sabah traditional rice varieties is very limited. Therefore we report a comprehensive genetic diversity and relationship study of 22 Sabah traditional rice varieties in three main divisions of Sabah including the West Coast Division (WCD) Sandakan Division (SD) and Interior Division (ID) using 11 random amplified polymorphic DNA (RAPD) markers. Our results showed that more than half of the collected rice seeds were medium in size and shape with moderately high head rice recovery and low moisture content. In addition about half of them were categorised with high to very high amylose content. Genetic analysis revealed a total of 75 bands were produced using all RAPD markers with 100 polymorphism and a high degree of genetic variation among all Sabah traditional rice varieties was obtained. The genetic differentiation of Sabahs traditional rice varieties was more likely to occur within divisions rather than among divisions. Furthermore Sabah traditional rice varieties in ID showed the greatest genetic diversity and polymorphic loci and were closely related to rice varieties in SD but genetically dissimilar to those in WCD as revealed in both phylogenetic tree and principal component analysis. In conclusion this study provides breeders with reliable information regarding diversity of Sabahs traditional rice varieties; the data could also be beneficial for local rice yield enhancement.


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Abstract

Sabah also known as North Borneo is one of the states in Malaysia. It is home to many local varieties of rice but the self-sufficiency quotient for rice production is only about 30. Knowledge of the genetic diversity of crops has been utilised to increase crop yields including rice in different countries but the information regarding the genetic diversity of Sabah traditional rice varieties is very limited. Therefore we report a comprehensive genetic diversity and relationship study of 22 Sabah traditional rice varieties in three main divisions of Sabah including the West Coast Division (WCD) Sandakan Division (SD) and Interior Division (ID) using 11 random amplified polymorphic DNA (RAPD) markers. Our results showed that more than half of the collected rice seeds were medium in size and shape with moderately high head rice recovery and low moisture content. In addition about half of them were categorised with high to very high amylose content. Genetic analysis revealed a total of 75 bands were produced using all RAPD markers with 100 polymorphism and a high degree of genetic variation among all Sabah traditional rice varieties was obtained. The genetic differentiation of Sabahs traditional rice varieties was more likely to occur within divisions rather than among divisions. Furthermore Sabah traditional rice varieties in ID showed the greatest genetic diversity and polymorphic loci and were closely related to rice varieties in SD but genetically dissimilar to those in WCD as revealed in both phylogenetic tree and principal component analysis. In conclusion this study provides breeders with reliable information regarding diversity of Sabahs traditional rice varieties; the data could also be beneficial for local rice yield enhancement.

Additional Metadata

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Item Type: Article
AGROVOC Term: Rice
AGROVOC Term: Oryza sativa
AGROVOC Term: Seeds
AGROVOC Term: Germplasm
AGROVOC Term: Random amplified polymorphic DNA
AGROVOC Term: Genetic diversity within species
AGROVOC Term: Genetic polymorphism
AGROVOC Term: Phylogeny
AGROVOC Term: Plant breeders
AGROVOC Term: Crops
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:29
URI: http://webagris.upm.edu.my/id/eprint/24861

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