Enhancement of the contents of anticancer bioactive compounds in mutant clones of rodent tuber (Typhonium flagelliforme Lodd.) based on GC-MS analysis

Sianipar Nesti Fronika, . and Purnamaningsih Ragapadmi, . Enhancement of the contents of anticancer bioactive compounds in mutant clones of rodent tuber (Typhonium flagelliforme Lodd.) based on GC-MS analysis. pp. 305-320. ISSN 1511-3701

Rodent tuber (Typhonium flagelliforme Lodd.) which is a well known herbal plant from the Araceae family is know for its anticancer activities. The genetic variation of rodent tuber is low due to its commonly applied vegetative propagation methods. Thus its genetic variation has to be increased to obtain a new and superior plant containing a high amount of anticancer compounds. The aim of this study was to analyse the chemical compounds of the leaves and tubers of rodent tuber mutant and non irradiated (control) plants by GC-MS method. In this study in vitro calli of rodent tuber was irradiated with 6 Gy of gamma ray which produced mutant plantlets which was genetially different from non irradiated (control) plants. Mutant plantlets had been acclimated and propagated in the greenhouse to obtain the 6th generation vegetative mutant clones (MV6) which are stable superior mutants. The results indicated that MV6 contained six new anticancer compounds in the leaves and four new anticancer compounds in the tubers which have not been detected in control. The new anticancer compounds present in leaves and tubers were identified by GC-MS. They are hexadecanoic acid methyl ester octadecadienoic acid phytol‚ gamma-sitosterol eicosane geranylgeraniol squalene octacosane and 7-pentadecyne. MV6 is the new superior variety and a potential source of anticancer drugs.

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Abstract

Rodent tuber (Typhonium flagelliforme Lodd.) which is a well known herbal plant from the Araceae family is know for its anticancer activities. The genetic variation of rodent tuber is low due to its commonly applied vegetative propagation methods. Thus its genetic variation has to be increased to obtain a new and superior plant containing a high amount of anticancer compounds. The aim of this study was to analyse the chemical compounds of the leaves and tubers of rodent tuber mutant and non irradiated (control) plants by GC-MS method. In this study in vitro calli of rodent tuber was irradiated with 6 Gy of gamma ray which produced mutant plantlets which was genetially different from non irradiated (control) plants. Mutant plantlets had been acclimated and propagated in the greenhouse to obtain the 6th generation vegetative mutant clones (MV6) which are stable superior mutants. The results indicated that MV6 contained six new anticancer compounds in the leaves and four new anticancer compounds in the tubers which have not been detected in control. The new anticancer compounds present in leaves and tubers were identified by GC-MS. They are hexadecanoic acid methyl ester octadecadienoic acid phytol‚ gamma-sitosterol eicosane geranylgeraniol squalene octacosane and 7-pentadecyne. MV6 is the new superior variety and a potential source of anticancer drugs.

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Item Type:	Article
AGROVOC Term:	Araceae
AGROVOC Term:	Herbaceous plants
AGROVOC Term:	Cancer (disease)
AGROVOC Term:	Vegetative propagation
AGROVOC Term:	Chemical compounds
AGROVOC Term:	Leaves
AGROVOC Term:	Tubers
AGROVOC Term:	Mutants
AGROVOC Term:	Gc (gas chromatography)
AGROVOC Term:	Genetic variation
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24869

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