Citation
Keong Bun Poh, . and Phua Wan Jien, . Characterization of phytochemical compounds and antimicrobial activity of crude alkaloid from papaya (Carica papaya L. var. Eksotika) leaf extract. pp. 667-676. ISSN 1511-3701
Abstract
The papaya plant yields a lot of by-products after its fruit is harvested. Papaya leaves contain a bio-active phytochemical that can serve as a potential source of natural antibacterial agents. Phytochemical screening has discovered that papaya leaf extract contains compounds such as alkaloids tannins and saponins. Crude alkaloid detection by Dragendorffs test was positive. Chemical structure characterisation of extracted crude alkaloids revealed by 1 H-NMR spectrum testing was partial as the spectrum identified only the chemical structure corresponding to aliphatic carbon compounds. Crude alkaloid dissolved in organic solvent dichloromethane showed no antifungal properties towards Candida albicans. Antibacterial properties of crude alkaloid dissolved in dichloromethane were selective on both Gram positive and negative bacteria. Kirby Bauer test results showed negative inhibition for both Staphylococcus aureus (Gram positive) and Salmonella typhi (Gram negative) but inhibition was positive for antibiotic resistant bacteria MRSA (Gram positive) and Escherichia coli (Gram negative) bacteria. Inhibition efficacy increased with the amount of crude alkaloids used. The present study supports the use of papaya by-products as an alternative natural antibiotic for both the community and healthcare personnel.
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Abstract
The papaya plant yields a lot of by-products after its fruit is harvested. Papaya leaves contain a bio-active phytochemical that can serve as a potential source of natural antibacterial agents. Phytochemical screening has discovered that papaya leaf extract contains compounds such as alkaloids tannins and saponins. Crude alkaloid detection by Dragendorffs test was positive. Chemical structure characterisation of extracted crude alkaloids revealed by 1 H-NMR spectrum testing was partial as the spectrum identified only the chemical structure corresponding to aliphatic carbon compounds. Crude alkaloid dissolved in organic solvent dichloromethane showed no antifungal properties towards Candida albicans. Antibacterial properties of crude alkaloid dissolved in dichloromethane were selective on both Gram positive and negative bacteria. Kirby Bauer test results showed negative inhibition for both Staphylococcus aureus (Gram positive) and Salmonella typhi (Gram negative) but inhibition was positive for antibiotic resistant bacteria MRSA (Gram positive) and Escherichia coli (Gram negative) bacteria. Inhibition efficacy increased with the amount of crude alkaloids used. The present study supports the use of papaya by-products as an alternative natural antibiotic for both the community and healthcare personnel.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Papayas |
AGROVOC Term: | Carica papaya |
AGROVOC Term: | Leaves |
AGROVOC Term: | Phytochemistry |
AGROVOC Term: | Antimicrobial properties |
AGROVOC Term: | Leaf extracts |
AGROVOC Term: | Tannins |
AGROVOC Term: | Alkaloids |
AGROVOC Term: | Saponins |
AGROVOC Term: | Nuclear magnetic resonance spectroscopy |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 24 Apr 2025 06:29 |
URI: | http://webagris.upm.edu.my/id/eprint/24903 |
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