Isolation and characterization of acid soluble collagen (ASC) and pepsin soluble collagen (PSC) extracted from silver catfish (Pangasius sp.) skin


Citation

Noor Mashitah Mohamed Hukmi, . and Norizah Mhd Sarbon, . Isolation and characterization of acid soluble collagen (ASC) and pepsin soluble collagen (PSC) extracted from silver catfish (Pangasius sp.) skin. pp. 2601-2607. ISSN 2231-7546

Abstract

The aims of this study are to isolate and characterize acid soluble collagen (ASC) and pepsin soluble collagen (PSC) extracted from silver catfish (Pangasius sp.) skin. Isolated ASC and PSC collagen were characterized in terms of chemical composition (moisture protein fat and ash content) protein concentration functional group solubility and morphological properties as compared to commercial collagen. Yields of ASC and PSC were 4.27 and 2.27 respectively. The chemical compositions of raw skin were 34.64 0.0281 0.0368 and 0.31 while the chemical compositions of ASC and PSC were 94.21 0.0348 0.0081 0.5915 and 88.25 0.0346 0.0092 and 29.24 for moisture protein fat and ash respectively. ASC and PSC had protein concentrations of 2.27 mg/mL and 2.70 mg/mL respectively. Functional group analysis revealed that both isolated collagens exhibited Amide A II and III as a fingerprint for collagen structure. The highest solubility was found at pH 4 for ASC pH 1 for PSC and pH 5 for commercial collagen. The morphology of the isolated collagens was porous and they contained fibril. In conclusion the characteristics of the isolated ASC and PSC from silver catfish (Pangasius sp.) skin indicate that value-added collagen can be produced from the alternative source of freshwater fish.


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Abstract

The aims of this study are to isolate and characterize acid soluble collagen (ASC) and pepsin soluble collagen (PSC) extracted from silver catfish (Pangasius sp.) skin. Isolated ASC and PSC collagen were characterized in terms of chemical composition (moisture protein fat and ash content) protein concentration functional group solubility and morphological properties as compared to commercial collagen. Yields of ASC and PSC were 4.27 and 2.27 respectively. The chemical compositions of raw skin were 34.64 0.0281 0.0368 and 0.31 while the chemical compositions of ASC and PSC were 94.21 0.0348 0.0081 0.5915 and 88.25 0.0346 0.0092 and 29.24 for moisture protein fat and ash respectively. ASC and PSC had protein concentrations of 2.27 mg/mL and 2.70 mg/mL respectively. Functional group analysis revealed that both isolated collagens exhibited Amide A II and III as a fingerprint for collagen structure. The highest solubility was found at pH 4 for ASC pH 1 for PSC and pH 5 for commercial collagen. The morphology of the isolated collagens was porous and they contained fibril. In conclusion the characteristics of the isolated ASC and PSC from silver catfish (Pangasius sp.) skin indicate that value-added collagen can be produced from the alternative source of freshwater fish.

Additional Metadata

[error in script]
Item Type: Article
AGROVOC Term: Pangasius spp
AGROVOC Term: Freshwater catfish
AGROVOC Term: Skin
AGROVOC Term: Pepsin
AGROVOC Term: Isolation techniques
AGROVOC Term: Extraction
AGROVOC Term: Chemical composition
AGROVOC Term: Moisture content
AGROVOC Term: Protein content
AGROVOC Term: Ash content
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:29
URI: http://webagris.upm.edu.my/id/eprint/24959

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