Optimisation of enzymatic directed interesterification of palm oil for high yield of triunsaturated triacylglycerol using response surface methodology

Lee Yee Ying, . and Miskandar Mat Sahri, . and Lai Oi Ming, . and Noor Lida Habi Mat Dian, . and Tan Chin Ping, . Optimisation of enzymatic directed interesterification of palm oil for high yield of triunsaturated triacylglycerol using response surface methodology. pp. 602-616. ISSN 1511-2780

Unsaturated fatty acids (USAFA) especially monounsaturated fatty acids (MUFA) has been shown to provide substantial health benefits. This study attempted to increase the triunsaturated (U3 ) triacylglycerols (TAG) content in palm oil (PO) via lipase-catalysed directed interesterification (EDIE) to enable the production of a liquid fraction that is high in USAFA especially MUFA. A response surface methodology with three factors and five levels in a central composite design was employed for optimisation of the EDIE conditions for the U3 TAG production. The effects of reaction temperature (20C-50C) reaction time (12-28 hr) and enzyme load (2-18) on the U3 TAG and by-products yields were investigated. Well-fitted quadratic and linear models were successfully established for both responses. All processing conditions affected significantly (p0.05) U3 TAG yield. By-products yield was affected by reaction time and temperature. Although increasing reaction temperature and enzyme load and extending the reaction time resulted in a higher U3 TAG yield a higher amount of by-products were formed as well. The optimised processing conditions were a reaction temperature of 30C and reaction time of 18 hr when immobilised lipase enzyme load was set at 10 (w/w) and resulted in yields of about 27 of U3 TAG and 23 of by-products.

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Abstract

Unsaturated fatty acids (USAFA) especially monounsaturated fatty acids (MUFA) has been shown to provide substantial health benefits. This study attempted to increase the triunsaturated (U3 ) triacylglycerols (TAG) content in palm oil (PO) via lipase-catalysed directed interesterification (EDIE) to enable the production of a liquid fraction that is high in USAFA especially MUFA. A response surface methodology with three factors and five levels in a central composite design was employed for optimisation of the EDIE conditions for the U3 TAG production. The effects of reaction temperature (20C-50C) reaction time (12-28 hr) and enzyme load (2-18) on the U3 TAG and by-products yields were investigated. Well-fitted quadratic and linear models were successfully established for both responses. All processing conditions affected significantly (p0.05) U3 TAG yield. By-products yield was affected by reaction time and temperature. Although increasing reaction temperature and enzyme load and extending the reaction time resulted in a higher U3 TAG yield a higher amount of by-products were formed as well. The optimised processing conditions were a reaction temperature of 30C and reaction time of 18 hr when immobilised lipase enzyme load was set at 10 (w/w) and resulted in yields of about 27 of U3 TAG and 23 of by-products.

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Item Type:	Article
AGROVOC Term:	Palm oils
AGROVOC Term:	High yielding varieties
AGROVOC Term:	Interesterification
AGROVOC Term:	Triacylglycerols
AGROVOC Term:	Unsaturated fatty acids
AGROVOC Term:	Lipases
AGROVOC Term:	Temperature
AGROVOC Term:	Enzymes
AGROVOC Term:	Byproducts
AGROVOC Term:	Screening tests
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/24985

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