Optimisation of biotransformation conditions of theaflavin-3’-gallate

Wu, P. and He, R. Y. and Chen, H. and Gan, Q. and Chen, Y. and Zhang, X. M. and Hu, T. and Li, S. M. (2024) Optimisation of biotransformation conditions of theaflavin-3’-gallate. International Food Research Journal (Malaysia), 31. pp. 387-397. ISSN 2231 7546

Theaflavin-3’-gallate was synthesised using polyphenol oxidase (PPO) from gallocatechin (EGC) and epicatechin gallonic acid (ECG). Using PPO, Box-Behnken design, and single factor test, the optimal reaction conditions were determined: ECG/EGC ratio of 3:7, magnetic stirring speed of 200 rpm, reaction temperature of 37°C, and enzyme concentration of 20 mg/100 mL. Under these conditions, the yield of TF-3'-G was 18.1%. These parameters represent the optimal conversion conditions for theaflavin-3’monogallate.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/31%20(02)%202024/10%20-...

Abstract

Theaflavin-3’-gallate was synthesised using polyphenol oxidase (PPO) from gallocatechin (EGC) and epicatechin gallonic acid (ECG). Using PPO, Box-Behnken design, and single factor test, the optimal reaction conditions were determined: ECG/EGC ratio of 3:7, magnetic stirring speed of 200 rpm, reaction temperature of 37°C, and enzyme concentration of 20 mg/100 mL. Under these conditions, the yield of TF-3'-G was 18.1%. These parameters represent the optimal conversion conditions for theaflavin-3’monogallate.

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Item Type:	Article
AGROVOC Term:	tea
AGROVOC Term:	polyphenols
AGROVOC Term:	plant products
AGROVOC Term:	enzymes
AGROVOC Term:	research
AGROVOC Term:	HPLC
AGROVOC Term:	chemicophysical properties
AGROVOC Term:	food processing
Geographical Term:	China
Uncontrolled Keywords:	theaflavin-3’-gallate, enzymatic reaction, conditions optimisation
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	23 Feb 2026 03:52
Last Modified:	23 Feb 2026 03:52
URI:	http://webagris.upm.edu.my/id/eprint/2592

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