Determination of Available Phosphorus In Soil Using ICP-OES And UV-VIS Spectrophotometer: A Comparison


Citation

Mohd Kamaruddin Jaffar, . and Nur Syafiqah Mohamad Sa’adan, . and Lee, Suan Chua (2024) Determination of Available Phosphorus In Soil Using ICP-OES And UV-VIS Spectrophotometer: A Comparison. Malaysian Journal of Soil Science (MJSS) (Malaysia), 28. pp. 255-267. ISSN 1394-7990

Abstract

This study aimed to establish an alternative approach to determine the amount of available phosphorus (P) in soil samples by employing inductively coupled plasma optical emission spectroscopy (ICP-OES) compared to the conventional Ultraviolet-Visible Spectrophotometry (UV-Vis). The UV-Vis method based on the absorbance of the blue phosphate-molybdate complex is time-sensitive because its colour intensity is increased with time. Meanwhile, ICP-OES offers fast measurement of multielement simultaneously in a single run. The results showed that the ICP-OES method has a stronger linear correlation, r of 0.999794 than the UV-Vis method, r of 0.9996. The results of ICP-OES were also reproducible over 10 consecutive days while the calibration of the UV-Vis method on the other hand started to plateau at 60 mg/L. The t-test showed that there is no significant difference between both methods with a p-value, of 0.7289 > 0.05. The ICP-OES method was more robust than the UV-Vis method as the ICP-OES method could cover a wide range of soil sample concentrations and had better sensitivity with lower IDL (0.0913 mg/L and 0.0941 mg/L, respectively), LOD (0.1005 mg/L and 0.1044 mg/L respectively) and LOQ (0.3045 mg/L and 0.3164 mg/L respectively). The precision for both methods was comparable with a relative standard deviation (RSD) of 8% indicating that the variability of the data was relatively low. Finally, proficiency testing results using the ICP-OES method showed satisfactory outcomes. In conclusion, the ICP-OES method approach is the best replacement for the UV-Vis method for the determination of available P in soil samples.


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Abstract

This study aimed to establish an alternative approach to determine the amount of available phosphorus (P) in soil samples by employing inductively coupled plasma optical emission spectroscopy (ICP-OES) compared to the conventional Ultraviolet-Visible Spectrophotometry (UV-Vis). The UV-Vis method based on the absorbance of the blue phosphate-molybdate complex is time-sensitive because its colour intensity is increased with time. Meanwhile, ICP-OES offers fast measurement of multielement simultaneously in a single run. The results showed that the ICP-OES method has a stronger linear correlation, r of 0.999794 than the UV-Vis method, r of 0.9996. The results of ICP-OES were also reproducible over 10 consecutive days while the calibration of the UV-Vis method on the other hand started to plateau at 60 mg/L. The t-test showed that there is no significant difference between both methods with a p-value, of 0.7289 > 0.05. The ICP-OES method was more robust than the UV-Vis method as the ICP-OES method could cover a wide range of soil sample concentrations and had better sensitivity with lower IDL (0.0913 mg/L and 0.0941 mg/L, respectively), LOD (0.1005 mg/L and 0.1044 mg/L respectively) and LOQ (0.3045 mg/L and 0.3164 mg/L respectively). The precision for both methods was comparable with a relative standard deviation (RSD) of 8% indicating that the variability of the data was relatively low. Finally, proficiency testing results using the ICP-OES method showed satisfactory outcomes. In conclusion, the ICP-OES method approach is the best replacement for the UV-Vis method for the determination of available P in soil samples.

Additional Metadata

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Item Type: Article
AGROVOC Term: soil analysis
AGROVOC Term: phosphorus
AGROVOC Term: soil fertility
AGROVOC Term: spectrophotometry
AGROVOC Term: chemical analysis
AGROVOC Term: ultraviolet radiation
Geographical Term: Malaysia
Depositing User: Mr. Khoirul Asrimi Md Nor
Date Deposited: 23 May 2025 02:53
Last Modified: 23 May 2025 02:53
URI: http://webagris.upm.edu.my/id/eprint/2826

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