Comparative detection of Lumpy Skin Disease Virus (LSDV) using Taqman real-time PCR and conventional PCR

Mohamad Azlan J., . and Nurain Izzati S., . and Nur Ain Shafiqah M. S., . and Tuba Thabitah A. T., . and Shahrol Z., . and Mazatonazuar M. I., . and Mohd Shafarin S., . (2023) Comparative detection of Lumpy Skin Disease Virus (LSDV) using Taqman real-time PCR and conventional PCR. Malaysian Journal of Veterinary Research (Malaysia), 14 (1). pp. 23-31. ISSN 2180-3897

Lumpy Skin Disease Virus (LSDV) is a contagious virus that affects cattle and has a considerable impact on animal health due to its potential for significant economic losses. The diagnosis of LSDV requires a fast, accurate, and feasible method in a veterinary laboratory to reduce the spread of the disease. The Polymerase Chain Reaction (PCR) has proven to be rapid and sensitive in detecting the DNA of the LSDV in different biological samples. This study aims to establish a PCR assay for the detection of LSDV and to compare the detection of this virus in clinical samples using the conventional PCR and Taqman real-time PCR. The primer or probe used is based on the OIE Terrestrial Manual 2018 Chapter 3.4.12 Lumpy Skin Disease. Detection of LSDV was conducted using PCR while the test specificity and sensitivity were determined using 121 clinical samples from cattle with clinical signs and highly suspected LSDV. The Taqman real-time PCR detection showed better sensitivity compared to conventional PCR when tested with a 10-fold dilution of the samples. While for specificity, the conventional PCR and Taqman real-time PCR are comparable. Among these 121 clinical samples, 69.42% (n=84) presented positive results by Taqman real-time PCR and 25.62 % (n=31) were detected as positive by conventional PCR. This study concludes that skin, scab, meat, and lymph nodes were better samples for Taqman real-time PCR with 100% positive results while whole blood in EDTA, nasal swab, and saliva swab samples gave more than 50% positive results. For conventional PCR, the best samples for LSDV identification were skin (100 %), scab (80.95%), and meat (42.85%). The results suggest that the Taqman real-time PCR technique serves as a better diagnostic tool of LSDV in cattle on a variety of samples. In conclusion, the use of Taqman real-time PCR for detection of LSDV is more accurate and robust, thus preferable for LSDV detection.

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Abstract

Lumpy Skin Disease Virus (LSDV) is a contagious virus that affects cattle and has a considerable impact on animal health due to its potential for significant economic losses. The diagnosis of LSDV requires a fast, accurate, and feasible method in a veterinary laboratory to reduce the spread of the disease. The Polymerase Chain Reaction (PCR) has proven to be rapid and sensitive in detecting the DNA of the LSDV in different biological samples. This study aims to establish a PCR assay for the detection of LSDV and to compare the detection of this virus in clinical samples using the conventional PCR and Taqman real-time PCR. The primer or probe used is based on the OIE Terrestrial Manual 2018 Chapter 3.4.12 Lumpy Skin Disease. Detection of LSDV was conducted using PCR while the test specificity and sensitivity were determined using 121 clinical samples from cattle with clinical signs and highly suspected LSDV. The Taqman real-time PCR detection showed better sensitivity compared to conventional PCR when tested with a 10-fold dilution of the samples. While for specificity, the conventional PCR and Taqman real-time PCR are comparable. Among these 121 clinical samples, 69.42% (n=84) presented positive results by Taqman real-time PCR and 25.62 % (n=31) were detected as positive by conventional PCR. This study concludes that skin, scab, meat, and lymph nodes were better samples for Taqman real-time PCR with 100% positive results while whole blood in EDTA, nasal swab, and saliva swab samples gave more than 50% positive results. For conventional PCR, the best samples for LSDV identification were skin (100 %), scab (80.95%), and meat (42.85%). The results suggest that the Taqman real-time PCR technique serves as a better diagnostic tool of LSDV in cattle on a variety of samples. In conclusion, the use of Taqman real-time PCR for detection of LSDV is more accurate and robust, thus preferable for LSDV detection.

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Item Type:	Article
AGROVOC Term:	cattle
AGROVOC Term:	lumpy skin disease
AGROVOC Term:	PCR
AGROVOC Term:	diseases
AGROVOC Term:	quantitative polymerase chain reaction
AGROVOC Term:	lymph nodes
AGROVOC Term:	blood
AGROVOC Term:	diagnostic techniques
Geographical Term:	Malaysia
Depositing User:	Mr. Khoirul Asrimi Md Nor
Date Deposited:	23 Jun 2025 01:46
Last Modified:	23 Jun 2025 01:46
URI:	http://webagris.upm.edu.my/id/eprint/2876

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